Spark Holland

In principle, on-line SPE-LC can be automated quite easily as well, for instance, by using Such programmable on-line SPE instrumentation as the Prospekt (Spark Holland) or the OSP-2 (Merck) which have the capability to switch to a fresh disposable pre-column for every sample. Several relevant applications in the biomedical field have been described in which these devices have been used. Eor example, a fully automated system comprising an autosampler, a Prospekt and an LC with a UV... 

Sample preconcentration was performed by means of an automated on-line SPE sample processor Prospekt-2 (Spark Holland, Emmen, The Netherlands). Oasis HLB cartridges (Waters, Barcelona, Spain) were used to preconcentrate cannabi-noids present in the water samples whereas isolation of the rest of the compounds was done in PLRPs cartridges (Spark Holland). Before extraction, influent samples were diluted with HPLC water (1 9, v/v) to reduce matrix interferences and to fit some analyte concentrations, e.g., cocaine (CO) and benzoylecgonine (BE), within the linear calibration range. A sample volume of 5 mL was spiked with the internal standard mixture (at 20 ng/L) in order to correct for potential losses during the analytical procedure, as well as for matrix effects. Elution of the analytes to the LC system was done with the chromatographic mobile phase. 

Commercially available fully automated online SPE systems include Prospekt, Prospekt-2, and Symbiosis systems (Spark Holland, the Netherlands) and Merck s OSP-2 (Darmstadt). Figure 10.2 shows a Symbiosis (Kuklenyik et al. 2005) two-cartridge, single analytical column online SPE... 

Parts of this document have been reproduced by kind permission of Spark Holland Company, the Netherlands. 

For online coupling of SPE with HPLC it is possible to use more automated equipment, such as the Prospekt (Spark Holland, Netherlands) or the OSP-2 (Merck, Darmstadt, Germany). Both systems have been validated for determining 11 OPPs at a level of 0.1 /rg/L or lower in water, with good results (49-52). 

Prospekt Spark Holland SPE Indirect/direct Serial Marketed by... 

Cartridge/plate Phenomenex Strata X 33 pm Spark-Holland HySphere ... 

We are grateful to P. Tiller (Merck and Co.), Y.-Q. Xia (Bristol-Myers Squibb), A. Watt (Merck and Co.), T. Pereira (Merck and Co.), Waters, Finnigan, Sciex/Apphed Biosystems, Beckman-Coulter, Spark-Holland, Tomtec, and LEAP Technologies for providing some of the hgures and helpful discussions. 

Until recently, on-line SPE via valve-switching techniques was not veiy popular in bioanalytical LC-MS. With the introduction of the Spark Holland Prospekt II, online SPE-LC-MS is promoted for quantitative bioanalysis. The typical procedure and column-switehing setup for on-line SPE-LC-MS is discussed Ch. 1.5.4. 

Figure 10.5. PROSPEKT automated solid-phase extraction system. (Photograph published with the permission of Spark Holland and Jones Chromatography.)...

Published with the permission of Spark Holland and Jones Chromatography. 

Figure 10.19. Chromatographic separation of phenylurea herbicides at 10 pg/L (Published with the permission of Spark Holland).

Spark Holland Apphcation Note 53074 (2007) (available at http //WWW.sparkholland.com/applications/appl ication-search accessed 11/22/10). 

In subsequent studies, the development and use of an automated benchtop instrument received much attention. The final system (see Fig. 3) consisted of a Prospekt (Spark Holland, Emmen, Netherlands) automated sample handling module for trace enrichment, drying of the SPE cartridge, and analyte transfer under PCSE conditions using an SVE and an on column injector, coupled on-... 

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