SPARC cysteine

CS-PG, chondroitin sulfate-proteoglycan these are similar to the dermatan sulfate PGs (DS-PGs) of cartilage ffable 48-11). SPARC, secreted protein acidic and rich in cysteine. 

SPARC (Secreted Protein Acidic and Rich in Cysteine), 46 484-485 Specific activity method, of isotope half-life determination, 2 326-327 Specific interaction theory, application, 43 19-21... 

Puolakkainen P, Bradshaw AD, Kyriakides TR, Reed M, Brekken R, Wight T, Bomstein P, Ratner B, Sage EH. Compromised production of extracellular matrix in mice lacking secreted protein, acidic and rich in cysteine (SPARC) leads to a reduced foreign body reaction to implanted biomaterials. American Journal of Pathology 2003,162, 627-635. 

Complete sequence analysis based on cDNA was achieved for osteonectin and SPARC and indicated a disulfide-bonded, four-domain structure for the protein (Fig. 12). Remarkable features of the structure are a cluster of 16 glutamic acid residues at the N terminus and an EF-hand domain located between the last two cysteine residues, both regions having the potential for calcium-binding (Bolander et al., 1988 Engel et al., 1987). Studies with BM-40 demonstrated a reversible change in a helix from 30 to 20% upon removal of calcium. The data indicate the... 

Fig. 12. Predicted domain structure of the small calcium-binding protein BM-40/os-teonectin/SPARC. Numbering is according to the sequence of SPARC (Mason et al., 1986a), but omits the signal peptide. A dashed line denotes predicted a helix, C identifies cysteine residues, and + indicates clusters of basic residues. A potential calcium-binding domain of the EF type is noted as well as a cluster of glutamic acids. Modified from Engel et al. (1987).

Bleomycin (0.15 U/mouse) given intratracheally induced significantly more pulmonary fibrosis in mice deficient in SPARC (secreted protein, acidic and rich in cysteine) compared with that in wild-type control mice, with the mutant mice demonstrating greater neutrophil accumulation in the lungs (Savani et al. 2000). However, in wild-type and SPARC-deficient mice given intraperitoneal bleomycin (0.8 U/injectionx 5 injections over 14 days), the pattern and severity of pulmonary fibrosis, as well as the levels of leucocyte recruitment, were similar in both strains of mice. 

Secreted protein, SPARC acid, cysteine rich... 

Secreted protein, acidic and rich in cysteine (SPARC) is a multifunctional glycoprotein found within the extracellular matrix in a variety of tissues. It was found to be consistently upregulated in microarray analyses of tissue from scleroderma patients, and three new SNPs were found by sequencing the gene in... 

A second category of NCPs with Ca-binding properties is classified as mineraUzed tissue specific, since they are found in all the calcified connective tissues, namely, dentin, bone, and cementum. These proteins include osteocalcin (OC) and bone sialoprotein (BSP). A serine-rich phosphoprotein called dentin matrix protein 1 (Dmp-1), whose expression was first described as being restricted to odontoblasts [5], was later shown to be expressed by osteoblasts and cementoblasts [6]. Other NCPs include osteopontin and osteonectin [secreted protein acidic and rich in cysteine (SPARC)]. The fourth category of dentin NCPs is not expressed in odontoblasts but is primarily synthesized in the liver and released... 

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