Source light microscopy

In 1994, we proposed that a metallic needle having a nano-tip at its apex be employed as a nano-light-source for microscopy attaining nanometric spatial resolution [2]. Later, we expanded the technique to Raman spectroscopy for molecular nano-identification, nano-analysis and nano-imaging. In this chapter, we give a brief introduction to local plasmons and microscopy using a metallic nano-needle to produce the local plasmons. Then, we describe the microscope that we built and... 

Transmission electron microscopy (TEM) has been used extensively in biology for direct visualization of ultrastructural details and platinum deposits in cells. The underlying physics behind TEM is similar to that of ordinary light microscopy however, the resolutions achieved by TEM can be some 400-fold greater than that of light microscopy (38). Briefly, the mechanics behind TEM involves an illuminating source, the electron gun, that sends a beam of electrons through a vacuum and onto the... 

Compare light microscopy, transmission electron and scanning electron microscopy in terms of optical arrangement, illumination source, working environment, imaging formation mechanism and specimen preparation discuss their similarities and differences. 

Bacterial flagella are too thin to be visualized by regular light microscopy (bright field, dark field, or phase contrast microscopy). However, if the microscopes light source is very strong, the flagella scatter sufficient... 

Figure 5.24 Schematic representation of carbon fiber structures obtained from Courtelle precursor, (a) Isotropic center—with an outside skin of oriented crystalline material, (b) Double cross— with the outside showing a different orientation to that of the center, (c) Single cross—where the complete fiber shows one type of preferred orientation. Source Reprinted from Knibbs RH, The use of polarized light microscopy in examining the structure of carbon fibres, J Microscopy, 94(3), 273-281, 1971.

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