Solution-phase synthesis chromatography

Monitoring reaction progress throughout a multistep synthesis is a relatively difficult task.22 Typical methods used for solution-phase synthesis, including thin-layer chromatography (TLC), GC, and most types of mass spectrometry (MS), are less informative for solid-phase methods. However, Fourier transform infrared (FTIR) spectroscopy and nuclear magnetic resonance (NMR) are particularly useful in solid-phase strategies. 

Bifunctionally tagged Mitsunobu reagents 21 and 22, quaternary ammonium carbonate resin 65, tetrafluorophthalic anhydride (as a solution-phase linking reagent), and amine-functionalized resin 2 were used in a three-step solution-phase synthesis of a series of substituted hydroxypiperidines.39 No further purification (e.g., liquid-phase extraction or chromatography) was required, and products were isolated in good yields and purities. 

The synthesis of the Saa-peptide conjugates in solution follows standard solution-phase synthesis using, for example, IIDQt117 or EDC as the coupling agent. The reaction is checked by TLC (ninhydrin and/or 10% H2S04 in MeOH dip). The product is usually purified by flash chromatography. 

Kallikrein Complex kallikrein/kininogen (enzyme/substrate) mimicking Phe-Arg dipeptide on the substrate Solution-phase synthesis Affinity chromatography 9... 

IgG Complex SpA-IgG (ligand/protein) mimicking the Phel32-Tyrl33 dipeptide on the natural ligand Solution-phase synthesis Affinity chromatography 4... 

Human recombinant Factor Vila Complex tissue factor/factor Vila ligands designed to bind to the Gla-domain in Factor Vila. Solid-phase combinatorial chemistry solution-phase synthesis of a sub-library Affinity chromatography SPR 7,15 ... 

Recombinant insulin precursor MB Study of the target protein per se and selection of appropriate binding sites Solid-phase comhinatorial chemistry (64 ligands) solution-phase synthesis of a suh-lihrary Affinity chromatography SPR 15,18,19... 

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