Solid-phase technology

A series of piperidine containing All activated C4-carboxy azetidinone tryptase inhibitors was prepared by Sutton et al. [213] adopting solid-phase technology. Wang resin was used to attach with the carboxyl group of the (5-lactam moiety and removal from the resin surface was achieved as usually by 20% TFA in CH2C12. 

H.G. Johnson, Recycling of particulate metal via solid phase technology, Proc. Inst. Briquet. Agglom. Bien. Conf., 14 (1975) 183—195. 

Because SPA is a solid phase technology, it is important to optimize the amount of receptor with respect to the immobilizing surface. The surface area of the... 

Based on solid phase technologies numerous fully automated immunoassay systems which allow the determination of a broad spectrum of analytes are nowadays commercially available. While very successful in the case of nonisotopic methods, attempts for automation in the RIA field more or less failed due to the properties of the radioactive label. 

Van den Brulle J, Fischer M, Langmann T, Horn G, Waldmann T, Arnold S, Fuhrmann M, Schatz O, O Connell T, O Connell D, Auckenthaler A, Schwer H (2008) A novel solid phase technology for high-throughput gene synthesis. Biotechniques 45 340-343... 

Another important discovery in the area of combinatorial synthesis with di- and tri-saccharides was made by Kahne and co-workers at Princeton [4c]. Kahne s approach utilized solid phase technology to synthesize a saccharide library (Scheme 3), which was especially challenging since, in order for a solid phase approach to be successful, bonds between monomers must be formed in high yields. This is not trivial since most high-yielding coupling reactions in carbohydrate chemistry are not general in nature [5]. 

At the end of the 1960s, the peptide community was elated by reports of the synthesis of ribonuclease A using both classical and solid-phase technologies. The classical approach was labor intensive. Both methods gave low overall yields of the desired protein however, these achievements were milestones in that a protein with enzymatic activity had been synthesized. Emil Fischer s dream had been realized. 

Chauhan, P.M. (2001) Recent development in the combinatorial synthesis of nitrogen heterocycles using solid phase technology. Combi. Chem. High Throughput Screening 4 35-51. 

Several more complex trisaccharides involving modified sugars have been prepared for example 1, which is an aureolic acid-related compound made from a 2-phenylthioglycosyl trichloroacetimidate derived from the glycal 2 by solid phase technology, and the C-3 trisaccharide 3 of antibiotic PI-080, the unsaturated unit of which was coupled using the relevant unsaturated glycosyl tetrazole derivative. ... 

Solid phase technology and use of 6-0-acetyl-2,3,4-tri-0-benzyl-a-D-glucosyl trichloroacetimidate led to (1 - 6)-linked pentasaccharides. ... 

PROBLEM 17.19 Write all of the equations for the synthesis of Gly—Ala—Phe using Merrifield solid-phase technology. 

Attention should be given to the similarity between solid phase technology and the biological synthesis of proteins. In both cases, an amino acid is attached via the carboxylate function to a large macromolecular surface upon which the sequential addition of other amino acids and peptide bond formation occurs. In one case, the polymer, like the tRNA molecule at the peptidyl site, is the leaving group, while in the other case, the polymer, like the tRNA molecule at the aminoacyl site, remains bound to the chain after formation... 

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