Solid controlled pore glass

Haller, W. (1983). Application of Controlled Pore Glass in Solid Phase Biochemistry (W. Haller, ed.), pp. 535-597. Wiley, New York. 

CPG = Controlled Pore Glass (solid support) 1 and 2 (Bj, B2, B3, B4), commercially available... 

Haller, W., Application of controlled pore glass in solid phase biochemistry, In W.H. Scouten, ed. Solid Phase Biochemistry New York Wiley, 1983 535-597. 

Van Zoonen et al. [19,20] employed an alternative approach, in an attempt to overcome the limited aqueous solubility of diaryloxalate ester-type POCL reagents. In this work, granular TCPO was mixed with controlled pore glass and packed in a flow cell, forming a solid-state TCPO reactor. When this was used in conjunction with a flow system, some of the TCPO dissolved in the carrier solution. Numerous difficulties were encountered with this approach, namely, limited reactor lifetime (approximately 8 h) and low CL emission obtained as the carrier became more aqueous (a 90% reduction of CL intensity occurred when the aqueous content of the carrier stream comprised 50% water, as compared to pure acetonitrile). The samples also required dilution with acetonitrile to increase the solubility of TCPO in the sample plug. 

Even controlled-pore glass (CPG) could be successfully employed as solid support with (9-glycosyl trichloroacetimidates as glycosyl donors. Thus, limitations of solvents and reaction temperatures in the glycosylation step, as experienced with the Merrifield resin, are restricted to those observed in solution-phase synthesis. Therefore, regio- and stereocontrol of the glycosylation reactions should be available from well-established solution-phase methodologies. 

In solid-phase syntheses, oligonucleotides are usually synthesized in the 5 -direction from an immobilized 3 -terminus. The solid phase is generally silica or controlled pore glass (CPG), which has been... 

A number of recent developments in 129Xe NMR spectroscopy are presented with direct applications to the study of mesopore space in solids. This includes the establishment of a relationship between pore size and chemical shifts for a number of controlled pore glasses and the exploration of hyperpolarized (HP) xenon for a number of NMR and microimaging applications to porous solids. With HP xenon, the increase in experimental sensitivity is remarkable. Experiments illustrated include the rapid characterization of the void space in porous solids, including the in-situ study of processes such as diffusion and dehydration, and imaging with chemical shift resolution. 

In this contribution we report an extension of a pore size - shift relationship for xenon in a number of controlled pore glasses (CPG s), and also report some applications of HP xenon spectroscopy to the study of void space in solids. 

For the automated solid-phase synthesis of oligonucleotides, however, silica was found to be the support of choice [192-197]. Silica with large pore size (25-300 nm), so-called controlled pore glass (CPG), is generally used for this purpose. The main advantages of CPG, as compared with silica gel, are its more regular particle size and shape, and greater mechanical stability. 

Chemical DNA-synthesis Another way to gain DNA is the chemical solid phase synthesis on controlled pore glasses (CPG), i.e. the phosphortriester method. The synthesis starts with a single nucleoside that is protected... 

Brunet F, Charpenrier T, Le Caer S, Renault JP. (2008) Solid-state NMR characterization of a controlled-pore glass and of the effects of electron irradiation. 

Heckel A, Mross E, Jung KH, Rademann J, Schmidt RR. Oligosaccharide synthesis on controlled-pore glass as solid phase material. Synlett 1998 2 171-173. 

The above list of solid supports is by no means exhaustive. Many others have been proposed and evaluated for use in peptide synthesis, including polypropylene membranes, cotton, controlled-pore glass beads, polyethylene sheets, and others. 

Kool reported the synthesis of macrocyclic nucleotide-hybrid compounds (215a-h), putative inhibitors of the HCV polymerase, polymerase C NS5B. The compounds were prepared by solid phase synthesis on controlled pore glass. 

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