Sodium laurylsulfate

It was found that the effect of solvents and various surfactants Triton X-100, Twin-80, Brij-35 sodium laurylsulfate, sodium cetylsulfate, cetylpyridinium chloride, cetyltrimethylammonium bromide on the luminescence intensity is insignificant. 

C12H25OSOi Na+ Sodium dodecylsulfate, SDS Sodium laurylsulfate, NaLS 8 x 1(T3... 

Utsumi, I., K. Kono, and Y. Takeuchi. 1973. Effect of surfactants on drug absorption. IV. Mechanism of the action of sodium glycocholate on the absorption of benzoylthiamine disulfide in the presence of sodium laurylsulfate and polysorbate 80. Chem Pharm Bull (Tokyo) 21 2161. 

Cleanser Triethanolamine laurylethersulfate, lauryl betaine Sodium Laurylsulfate, poloxamer 188... 

Sodium laurylsulfate silanized silicagel silanized silicagel... 

Fig. 8-44. Determination of sodium laurylsulfate in a nickel/ iron plating bath. - Separator column lonPac NS1 (10 pm) eluent 0.01 mol/L NH4OH/acetonitrile (72 28 v/v) flow rate 1 mL/min detection suppressed conductivity injection 50 pL sample (1 10 diluted).

FIGURE 39.6 Solubilization of chloramphenicol by surfactants. Squares, cetrimide black circles, sodium laurylsulfate empty circles, polysorbate 80 triangles, PEG 40 monostearate. 

The value of Tk increases as the amphiphile hydrophobic chain length increases. The Krafft points of the sodium salts of the classic amphiphiles (alkyl sulfates, sulfonates, and benzenesulfonates) are usually below room temperature. The Krafft point is a function of the counter-ion. Alkaline earth cations give higher Krafft points for sodium laurylsulfate, 7 k = 9°C the values for the calcium, strontium, and barium salts are 50, 64, and 105°C, respectively. 

Nonionic polyoxyethylene ethers Anionic sodium laurylsulfate Cationic quaternary ammonium compounds Bile salts... 

As described in the previous section, the addition of a surfactant produced remarkable stimulation during the synthesis of CoA. In the immobilized cell system, some surfactants showed similar stimulative effects. When the gel in which intact cells were trapped was treated with sodium laurylsulfate before the reaction, it was remarkably activated. An addition of sodium laurylsulfate to the reaction mixture with trapped Intact cells caused an increased accumulation of CoA. Similar observations have been reported by other workers. Franks (M) has reported that polyacrylamide gel-entrapped Streptococcus faecalls, which catalyzes the degradative conversion of arginine to putrescine, was activated by treating it with lysozyme. Chlbata l. (15) activated Escherichia coll cells with aspartase activity, which was trapped in polyacrylamide gel, by autolysis. 

Figure 7. Time course of CoA synthesis in the presence of CoA. (A). Synthesis from pantothenic acid-, the reaction teas carried out with 4 ftmol (a) or 2 funol (b) CoA. Other conditions were the same as those described in Figure 2(A), except for addition of 2 mg sodium laurylsulfate. The mixture without CoA (c) was used as a control run. (B) Syrtthesis from P-pantothernc acid the reaction conditions were the same as those described in (A) except that sodium pantothenate was replaced with P-pantotheruc acid. Symbols are the same as those in (A).

Figure 8. Synthesis of CoA on an immobilized cell column. (A) Single column system a substrate mixture composed of sodium pantotheruite (2,5 fimol/mL), cysteine (10 nmoUmL), ATP (15 fxmoHmL), magnesium sulfate (10 imol/mL), potassium phosphate buffer, pH 6.5 (150 nmol/mL), and sodium laurylsulfate (1 mg/mL) teas applied to a column (1 X 20 cm) of gel-entrapped dried cells. The reaction was carried out at 34°C with a flow rate of SV = 0.1-O.2 hr h (B) Separated column system a substrate mixture composed of sodium pantothenate (2.5 /imol/mL), ATP (7.5 ixmol/mL), magnesium sulfate (10 nmol/mL), potassium phospate buffer, pH 6.5 (150 fimol/mL), and sodium laurylsulfate (1 mg/mL) was applied to the top of the column (1 X 10 cm). Solution (about 20 mh) passed through the column at a flow rate of SV = 0.1-0.2 hr t was collected every day. To the solution (20 mL), 200 nmol cysteine and 150 fimol ATP were added, which was then reacted at the bottom of the column (1 X 10 cm) with a flow rate of SV = 0.1-0.2 hr to yield CoA. The reaction temperature was 34°C. Consumption of pantothenic acid was checked both at the top (b) and bottom (a) columns.

Sodium dodecylsulfate (SDS, sodium laurylsulfate) [151-21-3] M 288.4, m 204-207°. Prtrily this detergent by Soxhlet extraction with petroleum ether for 24 hours, followed by dissolution in acetone/MeOH/H20 90 5 5(v/v) and recrystallisation [Politi et al. J Phys Chem 89 2345 1985], It has been ptrrified by two recrystallisations from absolute EtOH, aqueous 95% EtOH, MeOH, isopropanol or a 1 1 mixture of EtOH/isopropanol to remove dodecanol, and dried ttrrder vacuum [Ramesh Labes J Am Chem Soc 109 3228 1987], SDS has also been piuified by repeatedly foaming whereby a 0.15% aqueous solution is made to foam and the foam is discarded, then the H2O is removed in vacuo and the residue is diluted to the required concentrations [see Cockbain McMirllen Trans Faraday Soc 41 322 1951, or by hqttid-liqttid extraction [see Harrold J Colloid Sci 15 280 1960. Dry it over silica gel. For DNA work it shottld be dissolved in excess MeOH passed through an activated charcoal colttrtm and evaporated until it crystallises out. 

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