Sodium cyanoborohydride toxicity

Add 10 pi of 5 M sodium cyanoborohydride in 1N NaOH (Aldrich) per ml of the macromolecule solution volume prepared in (1). Caution Highly toxic compound. Use a fume hood and be careful to avoid skin contact with this reagent. 

In a fume hood, add 10 pi of 5M sodium cyanoborohydride (Sigma) per ml of reaction solution. Caution cyanoborohydride is extremely toxic. All operations should be done with care in a fume hood. Also, avoid any contact with the reagent, as the 5M stock solution is dissolved in 1 N NaOH. If a higher pH buffer was used for the Schiff base formation, then adjust the solution to pH 7.5 before adding the cyanoborohydride. 

In a fume hood, dissolve 125 mg of sodium cyanoborohydride in 1ml water (makes a 2M solution). Caution Highly toxic compound handle with care. This solution may be allowed to sit for 30 minutes to eliminate most of the hydrogen-bubble evolution that could affect the vesicle suspension. 

In a fume hood, add 0.2 ml of 1 M sodium cyanoborohydride (Aldrich) to each ml of the protein/dextran solution. Mix well. Caution Cyanoborohydride is extremely toxic and should be handled only in well-ventilated fume hoods. Dispose of cyanide-containing solutions according to approved guidelines. 

Add sodium cyanoborohydride (Aldrich) to a concentration of 20 mg/ml. Caution Highly toxic Use a fume hood and avoid inhalation of dust or vapors. Seal the reaction vessel with parafilm. Do not use a rigid sealing cap, since cyanoborohydride will liberate hydrogen gas bubbles over time and may rupture the vessel. 

Incubate the reaction mixture for 120 min at 4°C. Sodium cyanoborohydride is extremely toxic and should only be handled in a fume hood while wearing appropriate personal protective equipment. Cyanoborohydride-containing solutions also contain cyanide and must be disposed of according to appropriate local regulations. 

Reductive amination of carbonyl compounds. Sodium cyanoborohydride has been the reductant of choice for this reaction, even though it is highly toxic (4, 448-449). Borane-pyridine is recommended as a nontoxic substitute. The reactions are conducted in acetic acid, in which this borane is fairly stable, and a co-solvent such as CH2CI2 or THE... 

A solvent-free reductive amination protocol for carbonyl compounds using sodium borohydride supported on moist montmorillonite KIO clay is facihtated by MW irradiation (Scheme 2.2-44) [129]. Traditionally, sodium cyanoborohydride [130], sodium triacetoxyborohydride [131], and NaBH4 coupled with sulfuric acid [132] are reagents used for the reductive amination of carbonyl compounds the use of corrosive acids or cyanide-based reagents results in toxic-waste generation. The... 

The reduction of alkyl hahdes has been important in many syntheses. Sodium cyanoborohydride in HMPA will reduce alkyl iodides, bromides, and tosylates selectively in the presence of ester, amide, nitro, chloro, cyano, alkene, epoxide, and aldehyde groups [118]. Tri-n-butyltin hydride will replace chloro, bromo, or iodo groups with hydrogen via a free radical chain reaction initiated by thermal decomposition of AIBN [119]. Other functionality such as ketones, esters, amides, ethers, and alcohols survive unchanged. The less toxic tris(trimethylsilyl) silane can be used similarly [120]. 

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