Small organics, analysis

K, V N Viswanadhan and J J Wendoloski 1998. Prediction of Hydrophobic (Lipophilic) lerties of Small Organic Molecules Using Fragmental Methods An Analysis of ALOGP and GP Methods. Journal of Physical Chemistry 102 3762-3772. 

Ghose, A. K., Viswanadhan, V. N., Wendoloski,).). Prediction of hydrophobic (lipophilic) properties of small organic molecules using fragmental methods an analysis of... 

He Y. and Lee H.K., Large-volume sample stacking in acidic buffer for analysis of small organic and inorganic anions by capillary electrophoresis, Anal. Chem. 71, 995, 1999. 

MS imaging is extensively used for biological applications, in analysis of tissues and small organisms, but its applications are in principle unlimited. Hence, it should be possible for applications in cultural heritage to become available soon. 

FIGURE 3.1 Comparison of atmospheric pressure ionization mass spectrometry and other widely used analytical methods for analysis of small organic molecules. 

NMR is a remarkably flexible technique that can be effectively used to address many analytical issues in the development of biopharmaceutical products. Although it is already more than 50 years old, NMR is still underutilized in the biopharmaceutical industry for solving process-related analytical problems. In this chapter, we have described many simple and useful NMR applications for biopharmaceutical process development and validation. In particular, quantitative NMR analysis is perhaps the most important application. It is suitable for quantitating small organic molecules with a detection limit of 1 to 10 p.g/ml. In general, only simple one-dimensional NMR experiments are required for quantitative analysis. The other important application of NMR in biopharmaceutical development is the structural characterization of molecules that are product related (e.g., carbohydrates and peptide fragments) or process related (e.g., impurities and buffer components). However, structural studies typically require sophisticated multidimensional NMR experiments. 

As polarity plays an important role in the analysis of small organic compounds such as pharmaceuticals (most organic compounds can be neutral), the introduction of MEKC resulted in a boom of applications in this area. In biomedical analysis with CE, MEKC is the technique of choice for the analysis of drugs in pharmaceutical preparations or in body fluids. Several groups of drugs have been analyzed with considerable success. A typical... 

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