Skin tissue regeneration layers

Multilayered materials are being developed for application in the heahng of compromised wounds. This system is based on sequential release of complementary bioactive components to enhance the activation of dermal tissue regeneration. Recently, a layer-by-layer approach was used to produce a 3D cell/fiber composed by nanofibers of blended PCL, type I collagen, and human skin cells (fibroblasts and keratinocytes). The assembled cell/nanofiber constructs, without or with keratinocyte layers, were cultured to form dermal substitutes or bilayer skin substitutes that were evaluated in nude mice (Mahjour et al., 2015). 

Since the biodegradable polymer scaffolds have a high surface area and porosity without the formation of a skin layer, they can be advantageously used for regenerating almost all living tissues and organs in tissue engineering (47). 

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