Resolvase/invertase family

The resolvase / invertase family and invertible DNA sequences. A second large family of recombinases act by cleaving a target DNA sequence hydrolytically leaving a free 3 -OH end (Eq. 27-14, step a). This free end then attacks a phosphodiester linkage in a second strand of DNA, cleaving that strand with an in-line nucleophilic displacement (step b). Active sites usually contain a characteristic cluster of aspartate and... 

Genetic modification of P. putida is highly relevant for metabolic engineering of producing strains. Modifications of interest consist of deletions and insertions attributing new features to the genome [66]. Such manipulations can be obtained by (i) homologous recombination (mediated by the RecA catalytic activity), (ii) site-specific recombination (subordinated to either the resolvase-invertase family or the FLP-fRT, Cre-loxP family, also referred to as the Int family), and... 

In vitro site-specific integration of bacteriophage DNA catalyzed by a recombinase of the resolvase/invertase family. Proc. Natl. Acad. Scl U.S.A.,... 

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