Imaging single molecules

Jasny J, Sepiol J, Irngartinger T, Traber M, Renn A and Wild U P 1996 Fluorescence microscopy in superfluid helium single molecule imaging Rev. Sc/. Instrum. 67 1425-30... 

Tokunaga M, Kitamura K, Saito K, Iwane A H and Yanagida T 1997 Single molecule imaging of fluorophores and enzymatic reactions achieved by objective-type total internal reflection fluorescence microscopy Biochem. Biophys. Res. Commun. 235 47-53... 

Lord SJ, Conley NR, Lee HD, Nishimura SY, Pomerantz AK, Willets KA, Lu Z, Wang H, Liu N, Samuel R, Weber R, Semyonov A, He M, Twieg RJ, Moemer WE (2009) DCDHF fluorophores for single-molecule imaging in cells. ChemPhysChem 10 55-65... 

We believe that single molecule imaging can contribute to a more thorough understanding of the role of pectin in the cell wall. Our future efforts will be focused on pectin gels formed in the presence of calcium. Eventually, it should be possible to visualize side chains on pectin and determine how rhamnose residues in the rhamnogalacturonan backbone of tobacco pectin disrupt the formation of helical regions. 

Seisenberger G, Ried MU, Endress T, Buning H, Hallek M, Brauchle C (2001) Real-time single-molecule imaging of the infection pathway of an adeno-associated virus. Science 294 1929-1932... 

C. Single-Molecule Imaging and Time-Resolved Fluorometry of TNP-ATP... 

The spatial and temporal progression of individual events involved in the formation of each type of structure can be monitored directly. A combination of imaging modes can be applied, each elucidating the process at a different length scale. Millimeter-scale variations can then be explained by nanometer-scale fluctuations. After the structures are built, single-molecule imaging can be employed to study catalytic reactions inside the nanopores. 

Single-molecule imaging techniques are not yet capable of monitoring several different chemical species simultaneously. 

Single-molecule imaging without fluorescence labeling... 

W. Trabesinger, A. Renn, B. Hecht, U. P. Wild, A. Montali, P. Smith and C. Weder, Single-molecule imaging revealing the deformation-induced formation of a molecular polymer blend, J. Phys. Chem. B 104, 5221 (2000). 

Y. Lill, K. L. Martinez, M. Lill, B. H. Meyer, H. Vogel and B. Hecht, Kinetics of the initial steps of G-protein coupled receptor mediated cellular signaling revealed by single molecule imaging, Chem. Phys. Chem. 6, 1633 (2005). 

G. Harms, L. Cognet, G. Blab, P. Lommerse, H. Kahr, R. Gamsjger, H. Spaink, N. Soldatov, C. Romanin and Th. Schmidt, Single-molecule imaging of L-type Ca2+ channels in live cells, Biophys. J. 81, 2639 (2001). 

P. H. M. Lommerse, G. A. Blab, L. Cognet, G. S. Harms, E. B. Snaar-Jagalska, H. P. Spaink and T. Schmidt, Single-molecule imaging of lipid-anchored proteins reveals domains in the cytoplasmic leaflet of the cell membrane, Biophys. J. 86, 609 (2004). 

DNA templated protein arrays with predictable control at the nanometer scale could lead to single-molecule detection in proteomics studies. Individual proteins placed at unique locations on the nanoarray could be detected with single molecule imaging techniques such as recognition imaging, in which specific antibodies are attached to the scanning probe cantilever. 

To many of us, a crystal structure is the most beautiful conceivable representation of a MIM because of its high content of truth nothing can be more accurate about the way a molecule looks - in the solid state at least - than an X-ray crystal structure, save perhaps for some very recent advances in single molecule imaging provided by atomic-resolution microscopy [81]. Although we cannot see the molecule itself, a crystal structure elicits the visualization of the exact positions of every atom and bond in a molecule relative to one another in the solid state. The ability to rotate and examine the structure from any angle in three dimensions supplies a satisfying sense of connection with the molecular world. 

Sako, Y, Minoghchi, S., and Yanagida, T. (2000). Single-molecule imaging of EGER signalling on the surface of living cells. Nat. Cell Biol. 2, 168-172. 

Webb SED, Needham SR, Roberts SK et al (2006) Multidimensional single-molecule imaging in live cells using total-intemal-reflection fluorescence microscopy. Opt Lett 31 2157-2159... 

Confocal Single Molecule Detection and Single Molecule Imaging... 

K. Adachi, H. Noji, K. Kinosita, Jr., Single-molecule imaging of rotation of Fi-ATPase. Meth. Enzymol. 361, 211-227 (2003). 

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