Single-molecule imaging, with

A. Single-Molecule Imaging with an Epi-Fluorescence Microscope... 

Here these techniques will be discussed from a general point of view and instructive examples will be presented. For didactic reasons, single-molecule imaging with a commercial epi-fluorescence microscope will be discussed... 

DNA templated protein arrays with predictable control at the nanometer scale could lead to single-molecule detection in proteomics studies. Individual proteins placed at unique locations on the nanoarray could be detected with single molecule imaging techniques such as recognition imaging, in which specific antibodies are attached to the scanning probe cantilever. 

To many of us, a crystal structure is the most beautiful conceivable representation of a MIM because of its high content of truth nothing can be more accurate about the way a molecule looks - in the solid state at least - than an X-ray crystal structure, save perhaps for some very recent advances in single molecule imaging provided by atomic-resolution microscopy [81]. Although we cannot see the molecule itself, a crystal structure elicits the visualization of the exact positions of every atom and bond in a molecule relative to one another in the solid state. The ability to rotate and examine the structure from any angle in three dimensions supplies a satisfying sense of connection with the molecular world. 

To transform cells with a plasmid vector carrying the gene of a target protein for single-molecule imaging, electroporation can be carried out by using a square pulse electroporator. 

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