Single-molecule imaging techniques

Single-molecule imaging techniques are not yet capable of monitoring several different chemical species simultaneously. 

DNA templated protein arrays with predictable control at the nanometer scale could lead to single-molecule detection in proteomics studies. Individual proteins placed at unique locations on the nanoarray could be detected with single molecule imaging techniques such as recognition imaging, in which specific antibodies are attached to the scanning probe cantilever. 

Single-Molecule Imaging Techniques to Visualize Chemotactic Signaling Events on the Membrane of Living DictyosteHum Cells... 

Miyanaga, Y., Matsuoka, S., Yanagida, T., and Ueda, M. (2007) Stochastic signal inputs for chemotactic response in Dictyostdium cells revealed by single molecule imaging techniques. Biosystems 88, 251-260. 

The ability of TIRFM to detect the positions and its changes of single molecules with high precision has been used to investigate motility of molecular motors. In fact, single molecule imaging techniques were first applied to molecular motors [11]. Myosin, kinesin, dynein and nucleotide polymerases are motor proteins that move along filamentous rail molecules such as F-actin, microtubule, and RNA or DNA. The first direct observation of the motility of mole-... 

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