Short-term mutagenicity assays

Thompson ED, McDermott JA, Zerkle TB, et al. 1989. Genotoxicity of zinc in 4 short-term mutagenicity assays. Mutat Res 233 267-272. 

The in vitro cytogenetic assay is a short-term mutagenicity test for detecting chromosomal damage in cultured mammalian cells. 

Although PBO had proved negative in the majority of short-term mutagenicity tests, the finding of a recent mouse oncogenicity study warranted investigation into the ability of PBO to produce DNA repair in cultured human liver slices, using the unscheduled DNA synthesis (UI)S) assay. Previous studies had shown that human liver slices were suitable for use in such studies of 01) (Beamand et at.. 1994, 1995). The effects of PBO on UDS in cultured human... 

Venitt, S. and Crofton-Sleigh, C. (1981). Mutagenicity of 42 coded compounds in a bacterial assay using Escherichia coli and Salmonella typhimurium. In Evaluation of Short-term Tests for Carcinogens. Report of the International Collaborative Program. Progress in Mutational Research (de Serres, F.J. and Ashby J., Eds.). Vol 1. Elsevier, New York, pp. 351-360. 

Several short-term bioassay procedures (3-14) have been developed recently which are applicable to detecting mutagenic and potential carcinogenic activity of organic substances. The SaimoneJla/mammalian mlcrosome assay or Ames Test (13-13] has been the most frequently applied and its efficacy has been well documented. This assay has also been applied to complex mixtures (19-22) to reduce greatly the time... 

In a number of short-term assays, DMF was not mutagenic or genotoxic. Inconsistent results have been reported in human in vivo studies for sister chromatid exchange and chromosomal aberration frequencies. ... 

IARC (1989) statement that, with respect to results from any one specific short-term assay, The relative potency of agents in tests for mutagenicity and related effects is not a reliable indicator of carcinogenic potency. ... 

Fox, M. Delow, G.F. (1985) Tests for mutagenic activity at the HGPRT locus in Chinese hamster V79 cells in culture. In Ashby, J., de Serres, F.J., Draper, M., Ishidate, M., Jr, Margolin, B.H., Matter, B.E. Shelby, M.D., eds, Progress in Mutation Research, Volume 5, Evaluation of Short-Term Tests for Carcinogens. Report of the International Programme on Chemical Safety s Collaborative Study on in vitro assays, Amsterdam, Elsevier Science, pp. 517-523... 

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