Shimadzu series

Shimadzu Xex = 486nm, A,em = 516 nm) with a chart recorder (Fisher Recordall, Series 5000, Springfield, NJ). F-PHEA concentrations in perfusate were determined by area comparison of the eluted curves to that of the standard. Curves of logMW vs. elution volume, Ve, were constructed for each column using the integral-MWD method as described previously (9). Molecular weight distributions of absorbed material were determined from the calibration curves and the sample s chromatograph by curve summation (9). 

For gas chromatography analysis, samples were spiked with 2-methyl-naphthalene as an internal standard. Samples were analyzed using a Shimadzu GC-17A series gas chromatograph equipped with RTX-5 column, 15 m (length) 0.25 mm (i.d.) and 0.25 pm (film thickness). The initial column temperature was 70 °C and temperature was increased at 20 °C min 300 °C, and column temperature was held for 13 min. Retention times R naphthalene, 3.2 min 2-methyl-naphthalene, internal standard, 4.09 min 1-tetralone, 4.7 min menadione, 5.68 min 1-naphthol, 5.7 min 4-hydroxy-1-tetralone, 6.1 min and 2-methy 1-4-hydroxy-1-tetralone, 6.18,6.27,6.3 and 6.4 min. 

FIGURE 2 Examples of modular and integrated HPLC systems. Upper row (I to r) modular systems Agilent 1100 Series, Jasco LC-2000 Series Lower row (I to r) integrated systems Waters Alliance, Shimadzu LC-2010. 

LC-MS/MS System Used and Analytical Conditions A Shimadzu liquid chromatography system was used (Shimadzu Scientific Instruments, USA). The system consisted of a Shimadzu SCL-10A VP system controller with four LC-10AD VP pumps. A Perkin-Elmer series 200 autosampler, equipped with a cool-tray (Perkin Elmer, Norwalk, CT, USA) was used for sample injection. Moreover, a six-port switching-valve was implemented in order to switch between an extraction column and the analytical column. Samples were injected onto the extraction column (Oasis HLB column). After a short extraction/equilibration time of 0.3 min, the samples were eluted from the extraction column onto the analytical column (Symmetry C18) (Figure 1). 

Figure 4.2. Examples of modular and integrated HPLC systems. Modular systems (a) Agilent 1100 Series, (b) Jasco low-pressure mixing system. Integrated systems (c) Waters Alliance, (d) Shimadzu 2010.

Modem Quadrupole GC/MS instrument used for the measurements in this book. Shimadzu GC-17A and QP-5000/5050A series... 

Countercurrent Chromatography Procedure. The entire column (pair of coiled multilayer columns connected in series) was filled with the stationary phase. The apparatus was then rotated counterclockwise at 600 rpm in planetary motion while the mobile phase was pumped into the inlet of the column at a flow-rate of 2.2 mL/min (head to tail elution mode). Maximum pressure at the outlet of the pump measured 80 psi. After a 1-hour equilibration period, the sample was loaded into the Rheodyne injector loop and injected. Effluent from the outlet of the column was continuously monitored with a Shimadzu UVD-114 detector at 312 nm and fractions collected with a Gilson FC-lOO fraction collector to obtain approximately 8.8 mL of eluant in each tube (during a 4-min interval). Retention of the stationary phase was estimated to be 930 mL (74%) by measuring the volume of stationary phase eluted from the column before the effluent changed to mobile phase (330 mL) and subtracting this volume from the total column capacity of 1260 mL. 

Chromatographic Separation The LC system was a Shimadzu (Columbia, MD) series lOAD VP equipped with binary pumps, a degasser, and an SIL-HT autosampler. A Betasil silica column (50 x 3 mm, 5 pm) from Keystone Scientific (Bellefonte, PA) operated under HILIC mode was used. The isocratic mobile phase was composed of 5 95 (v/v) A B, where A was 0.05% trifluoroacetic acid (TFA) in water (v/v) and B was 0.05% TFA in acetonitrile (v/v). The flow rate was 0.6 mL/min and the injection volume was 10 pL. Separation was performed at ambient temperature. 

Thermal Analysis Instrument 30 Series., Shimadzu, Kyoto. 

Shimadzu, Stand-Alone Thermal Analysis Instruments. Data Series of Novel Application. 

When a Shimadzu GC-16A series gas chromatograph is used as the core, various laboratory automation-orientated attachments snch as barcode and magnetic card readers become compatible a labour-saving system can be bnilt, in which the best operational parameters are automatically set. Each satellite gas chromatograph (GC-14A series) operates as an independent instrument when a keyboard nnit is connected. 

Shimadzu offers the TGA-50/50H with 1 g sample capacity and the TGA-51/51H with 10 g sample capacity. All systems have bottom-loading balances. Standard systems heat from ambient to 1000 °C, while the H systems heat from ambient to 1500 °C. All four systems can heat from 0.002 to 100°C/min and support vacuum to 10 torr. The TGA-50 series has smaller volume and... 

A Beckman series of liquid chromatograph equipped with a Model I65 variably wavelength detector, a Modell II4 solvent delivery pump, a Model 420 controller and a Model C-R3A Shimadzu integrator was used to perform the chromatographic separation of carotenoids, tocopherols and organic acid under different conditions which are shown in the figures or tables. 

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