Serum esterases

Aspirin serum esterase activity is species dependent.194 In man it is sex linked since it was found to be higher in men than women.195 It also seems to be age dependent.196... 

Godin SJ, Crow JA, Scollon EJ, Hughes MF, DeVito MJ, Ross MK (2007) Identification of rat and human cytochrome p450 isoforms and a rat serum esterase that metabolize the pyrethroid insecticides deltamethrin and esfenvalerate. Drug Metab Dispos 35 1664-1671... 

W. N. Aldridge, Serum Esterases. 1. Two Types of Esterases (A and B) Hydrolysing p-Nitrophenyl Acetate, Propionate and Butyrate, and a Method for Their Determination , Biochem. J. 1953, 53, 110-117 W. N. Aldridge, The Esterases Perspectives and Problems , Chem.-Biol. Interact. 1993, 87, 5-13. 

D. J. Stewart, T. Inaba, M. Lucassen, W. Kalow, Cocaine Metabobsm - Cocaine and Norcocaine Hydrolysis by Liver and Serum Esterases , Clin. Pharmacol. Ther. 1979, 25, 464-468. 

T. Ikeda, Two Prodrugs Activated by Serum Esterases Including Albumin , in Serum Albumin and rAcid Glycoprotein From Basic Sciences to Clinical Applications , Eds. M. Otagiri, Y. Sugiyama, B. Testa, J. P. Tillement, University of Kumamoto, 2001, p. 173-180. 

Wilson, I.B, 1955. Reactivation of human-serum esterase inhibited by alkylphosphates. J. Am. Chem. Soc. 77 2383-2386. 

Other earlier analyses made by Hansch et al. are represented by Eq. 30 and 31. Hydrolysis of p-nitrophenyl alkanoates (13) with serum esterase 25) ... 

In experiments on patients with breast cancer, it was found that up to a two-fold increase in serum /3-D-glucuronidase followed surgery, pregnancy, prolonged estrogen or cortisone therapy, etc.206 The /3-D-glucu-ronidase rise was usually accompanied by a fall in serum esterase, in both animal and human experiments. A rise in urinary ketosteroid excretion usually paralleled the changes, in man. 

Van Zutphen LFM (1974) Serum esterase genetics in rabbits. I. Phenotypic variation of the prealbumin esterases and classification of atropinesterase and cocainesterase. Biochem Genetics 12 309-326... 

MODIFICATIONS OF THE METHOD It can be appropriate to coincubate the compound of interest in the presence of inhibitors of serum esterases. Used is sodium fluoride, physostigmin or ecothiophate iodide (Chien 1990 Quon et al. 1993). In case of carboxy- or aminopeptidase cleavage of peptides specific peptidase inhibitors like amastatin, bestatin, phenylmethylsulphonylfluoride, 1,10-phenanthroline or ethylenediamine tetra acetic acid (EDTA) are useful (Lee 1995). 

New data from our experiments on enzyme changes with other chemicals now show that in rats, not only carbon tetrachloride but also ethylene dichloride, benzene, and methanol bring about a reduction in serum B-esterase during 4 days after a single oral dose at the acute level. These findings seem to indicate that the serum esterase activity response is considerably independent of the chemical structure and the molecular configuration of the stressing chemical, except that the stimulation of esterase activity occurs later than with the chlorinated hydrocarbon pesticides. 

Koitka, M. et al. Determination of rat serum esterase activities by an HPLC method using s-acetylthiocholine iodide and p-nitrophenyl acetate. Anal. Biochem. 2008, 381, 113-122. 

IPC proved valnable for estimating peptide hydrophobicity [13]. Pharmaceutical science utilized IPC to monitor rat serum esterase activities [14] and also to analyze relationship between plasma concentrations at the end of infusion and toxicity profiles of fixed-dose-rate gemcitabine plus carboplatin [15]. An IPC trap was also used in an online desalting-mass spectrometry system. This system allows ionic compounds in a nonvolatile buffer to be introduced into a MS for strutural elucidation. The trap column was equilibrated with a volatile IPR, the target analyte and the nonvolatile buffer ions (phosphate and sodium ions) were transferred into the trap column, but only the target analyte that interacts with the volative IPR can be retained phospahte buffer ion were eluted from the trap column and the target analyte was eluted by oragnic solvent in a backflush mode and introduced into the MS. 

Aldridge, W.N. (1953). Serum esterases. I. Two types of esterases (A and B) hydrolysing p-nitrophenyl acetate, propionate, butyrate and a method for their determination. Biochem. J. 53 110-17. 

Stewart, D.J. Inaba, T. Eucassen, M. and Kalow, W. Cocaine metabolism Cocaine and norcocaine hydrolysis by liver and serum esterases. Clin Pharmacol Ther 25 464-468, 1979. 

Cocaine and norcocaine hydrolysis by liver and serum esterases. Clin Pharmacol TAcr 25 464-468, 1978. 

Tl. Talal, N., Hermann, G., and deVaux St. Cyr, C., The immunological and chromatographic analysis of mouse serum esterases evidence for the molecular heterogeneity of serum albumin. Protides Biol. Fluids, Proc. CoUoq. 10, 183 (1963). 

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