Sequencing methods applications

A review containing 32 references has been published on sequencing methods applicable in the structural analysis of oligosaccharides. A further review by Whitesides and coworkers has dealt with the enzymic in vitro synthesis of mono-, oligo- and poly-saccharides, and the effects of several parameters on the yields and selectivities of glycosidase-catalysed formation of oligosaccharide glycosides have been discussed. ... 

Needleman S B and C D Wunsch 1970. A General Method Applicable to the Search for Similarities in the Amino Acid Sequences of Two Proteins. Journal of Molecular Biology 48 443-453. 

Research users need full access to the functional elements of the spectrometer system and require the most efficient and flexible tools for MR sequence and application development. If the measurement methods delivered with the software do not adequately address the specific investigational requirements of a research team, modem NMR software is an open architecture for implementing new and more sophisticated functionality, with full direct access to all hardware controlling parameters. After evaluation, the new functionality can be developed with the help of toolbox functions that allow rapid prototyping and final builds, to enable the new sequence to be executed by non-experienced personnel and then used in routine applications. These toolboxes provide application oriented definitions and connect to standard mechanisms and routine interfaces, such as the geometry editor, configuration parameters or spectrometer adjustments. 

Within each portfolio, the sequence of application of the various analytical methods is critical, as many methods have been validated with the assumption that all prior steps have been followed. For feedstocks, the sequence is sample preparation, then removal of nonstructural... 

Needleman, S. B. Wunsch, C. D. (1970). A general method applicable to the search for similarities in the amino acid sequences of two proteins. J Mol Biol 48,443-53. 

Furthermore, it has to be possible to determine the exact structure of a single compound within a library. As each compound in a combinatorial library is synthesized on a picomole scale, standard analytical methods are usually not applicable. These days, small amounts of peptides can be directly analyzed and identified using sequencing methods, but usually the compounds of a library... 

The same sequence is applicable to ketones, but in this case TiCU is superior to ZnBr2 as the catalyst. The method can be used with both cyclic and acyclic ketones and is applicable to both kinetic and thermodynamic silyl enol ethers for control of regiospecificity. ... 

The spectacular success of gene sequencing projects is largely due to the application of automated sequencing methods using laser-induced fluorescence techniques. The automation refers to the read-out process the preparation and loading of... 

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