Sequence assembly tools

Sequence assembly tools The iterative sequence alignment by incorporating the matched consensus sequence from the previous search into the subsequent round of search is called sequence assembly. The available tools are Staden assembler, TIGR assembler, and so on. 

Because all predicted genes receive unique database identifiers, which can be used, e.g., in your assembly tool, you can go through several annotation rounds following the progress of the draft genomic sequence without loosing previous information. 

The development of DNA sensors and high-density DNA arrays has been prompted by the tremendous demands for innovative analytical tools capable of delivering the genetic information in a faster, simpler, and cheaper manner at the sample source, compared to traditional nucleic acid assays. Nanoparticle-biopolymer conjugates offer great potential for DNA diagnostics and can have a profound impact upon bioanalytical chemistry. Nanoparticle/polynucleotide assemblies for advanced electrical detection of DNA sequences have been reviewed by Wang [145]. 

The innate sensitivity of DNA-mediated CT to perturbations in the TT-stack has prompted us to employ this chemistry as a probe of stacking structure and dynamics. We have developed a new class of DNA-based diagnostic tools that diagnose DNA mutations such as single base-pair mismatches and lesions, analyze DNA-protein interactions, and probe the sequence-depen-dent dynamics and flexibility of DNA. These applications rely on electrochemical probing of CT in DNA films self-assembled on gold electrodes. 

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