Sendai virus production

Human B-lymphoblastoid cell line Namalwa is derived from a Burkitt s lymphoma tumour biopsy and can be grown for an indefinitely long period of time (Klein et al., 1979 Biliau et ah, 1973). Although they contain Epstein-Barr (EB) nuclear antigen, Namalwa cells do not release EB virus. The cell line is used for the production of IFN-a. Namalwa cells can be induced to produce IFN by adding Sendai virus to cells pretreated with 2 mM sodium butyrate (Baker et al, 1980 Johnston, 1980) and can be primed with Sendai virus in order to increase the levels of IFN-a produced. 

Interferon Alfa-n3 (injection). Interferon alfa-n3 (Alferon N) is a polyclonal mixture of up to 14 natural a-interferon subtypes and contains 166 amino acid residues. Its commercial production involves induction of pooled units of human leukocytes with an avian virus (Sendai virus). The purification prtK css involves immunoaffinity and nitration chromatography. It is indicated primarily by intralcsional injection for the treatment of genital wans. The solution should be stored at a temperature of. 36 to 46°H and should not be shaken. 

Takahashi, T., Ryan, K. W., and Portner, A., 1992, Expression of cDNA encoding the Sendai virus hemagglutinin-neuraminidase gene Characterization of wild-type mutant gene products. Virology 187 837-840. 

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