Seminal vesicles acid phosphatase

As long ago as 1935, Kutscher and Wolbergs (K12) observed that semen and the prostate are among the richest sources of acid phosphatase in the human body. In a more recent survey (Bll) the acid phosphatase activities of seminal plasma in various species, determined as milligrams of nitrophenol liberated by 100 ml seminal plasma from 0.006 M p-nitrophenyl phosphate, in 60 minutes at 37°C and pH 4.9 were human, 274,000 cock, 15,000 turkey, 4000 bull, 570 rabbit 85. Human seminal plasma is made up by the secretory fluids produced in the epididymides, vasa deferentia, ampullae, seminal vesicles, the prostate and the bulbourethral (Cowper s) and urethral (Littre s) glands (M4). The semen contains many particulate bodies. Best known, of course, are the spermatozoa, which are formed in the seminiferous... 

On the basis of the specific biochemical characteristics of G-6-Pase, it was possible to show that this enzyme is located in cellular r ions different from those in which acid or alkaline phosphatases are found (Chiquoine, 1953). Similar histochemical procediu s were used recently in studying the seminal vesicles and prostate gland of the rat (Kuhlman, 1954). It was shown that tissues of the male accessory glands are capable of hydrolyzing both G-6-P and F-6-P. The histochemical reaction demonstrated in this... 

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