Self-assembly monolayer imprinted

Chen ZD, Nagaoka T (2000) Recognition of vitamin K1 with a molecularly imprinted self-assembled monolayer film. Bunsekikagaku, 49(7) 543-546... 

Self-assembled monolayer and layer-by-layer approaches were combined by thermal cross-linking on the surface of a silver electrode in order to develop a MIP-based sensor for the chiral analysis of thyroxine, a thyroid hormone with an amino-acid side chain [53]. The DPASV response peak current was linear to the concentration of thyroxine in the range from 0.010 to 17.2 ng mL" and the achieved detection limit was 0.0060 ng mL. The molecularly Imprinted film displayed absolute enantioselectivity to the template enantiomer and was applied for the determination of thyroxine in aqueous, blood and pharmaceutical samples with no interference from related compounds. 

Zhang, X., Huang, X., Lv, Z., 2013. Creating protein-imprinted self-assembled monolayers with multiple binding sites and biocompatible imprinted cavities. J. Am. Chem. Soc. 135, 9248-9251. 

A broader range of sensor devices has been developed, where the sensor response is modulated by the presence of an imprinted polymer, or an imprinted surface. Perhaps the simplest way of preparing an imprinted surface is in the use of self-assembled monolayer (SAM) techniques. A cholesterol-specific SAM was prepared by Piletsky et al. [44], in which a gold electrode was treated with a solution of cholesterol and hexadecanethiol in methanol. After the SAM had been established, the template was removed, leaving cholesterol-shaped cavities in the imprinted surface. Amperometric measurements were then used to investigate the sensitivity of the electrode towards cholesterol. It was found that the unmodified electrode, and the imprinted electrode displayed the same peak current. However, exposure of the imprinted electrode to a solution of template caused a decrease in... 

There are different methods to prevent the nonspecific adsorption of proteins onto surfaces, for example by adding surfactants or bovine serum albumin (BSA) to protein solutions, or by the widely applied method using self-assembled monolayers (SAMs) that are protein-resistant , such as SAMs with oUgo(ethylene glycol), l.e. OEG chains. These SAMs prevent nonspecific interactions due to their loose packing and the weU-hydrated nature. Such molecularly imprinted P-CD polymers, i.e. the P-CD molecular printboards were used as a platform for the immobilization of proteins through small multivalent orthogonal linker molecules. 

---