Protein onto surfaces

The adsorption of proteins onto surfaces is the oldest and easiest immobilization method. Adsorbing forces can be of different types Van der Waals interactions, ionic, hydrophobic or hydrogen bonding. The main advantages of this procedure are the simplicity of preparation and the little... 

Prime KL, Whitesides GM (1993) Adsorption of proteins onto surfaces containing end-attached oligo(ethylene oxide) A model system using self-assembled monolayers. J Am Chem Soc 115 10714-10721... 

Chiu, D.T., et al.. Patterned deposition of cells and proteins onto surfaces by using three-dimensional microfluidic systems. Proc. Natl. Acad. Sci. USA 2000, 97 (6), 2408-2413. 

Multiple inking is possible using microfluidic networks over the PDMS stamps.Papra, A. Bernard, A. Juncker, D. Larsen, N. B. Michel, B. Delamarche, E. Microfluidic Networks Made of Poly(Dimethylsiloxane), Si, and Au Coated With Polyethylene Glycol for Patterning Proteins Onto Surfaces. Langmuir 2001, 17, 4090-4095... 

Adsorption of proteins onto surfaces involves a complex interplay of reversibility, exchange, conformational change, irreversible attachment, and denaturation. Several of these processes may be followed by measurement of the circular dichroism of adsorbed species and of desorbed material. The profile of chromatographically adsorbed material also contains, in principle, the details of the complex kinetics. Some aspects of each of these processes are examined. 

Papra A, Bernard A, Juncker D, Larsen NB, Michel B, Delamarche E (2001) Microfluidic networks made of poly(dimethylsiloxane), Si, and Au coated with polyethylene glycol for patterning proteins onto surfaces. Langmuir 17 4090-4095... 

A simple procedure to pattern proteins onto surfaces was developed. The carbohydrate-based amphiphilic diblock copolymer, poly(styrene-Woc -[2-(p-D-galactosyloxy)ethyl methacrylate-co-styrene] [PS-fi-P(GalEMA-co-S)], was used for micellization and the production of honeycomb-stmctured porous films. Lectin binding assays using UV-vis spectroscopy and DLS showed that fluorescent PNA was successfully conjugated with the sugar moieties on the micelles and inside the pores of the porous films (Fig. 4) [62]. 

There are different methods to prevent the nonspecific adsorption of proteins onto surfaces, for example by adding surfactants or bovine serum albumin (BSA) to protein solutions, or by the widely applied method using self-assembled monolayers (SAMs) that are protein-resistant , such as SAMs with oUgo(ethylene glycol), l.e. OEG chains. These SAMs prevent nonspecific interactions due to their loose packing and the weU-hydrated nature. Such molecularly imprinted P-CD polymers, i.e. the P-CD molecular printboards were used as a platform for the immobilization of proteins through small multivalent orthogonal linker molecules. 

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