Self-assembled monolayers, reactive surfaces

Coupling of affinity molecules to surfaces also can be enhanced by the use of discrete PEG linkers. Nishimura et al. (2005) modified an amino surface with a NHS-PEG -maleimide crosslinker to create a hydrophilic self-assembled monolayer (SAM) surface that was thiol reactive for the conjugation of sulfhydryl-modified RNAs. This array then was used to investigate the binding specificity of synthetic kanamycins with selected RNA sequences to prove the specific interaction of ribosomal RNA with this molecule. The PEG linkers on surfaces provide lower nonspecific binding character than alkyl linkers, when preparing SAM surfaces for affinity interactions. 

Mrksich and co-workers developed a MALDI-based assay scheme making use of a target surface modification by self-assembled monolayers (SAMs) [22]. This combination of SAMs and MALDI is predominantly called SAMDI (selfassembled monolayers for MALDI). For SAMDI, a self-assembled monolayer with reactive end groups is used in order to covalently bind enzyme substrates to a surface. To... 

In light of these constraints, an effective chemical modification for anti-stiction treatments involves the application of a molecular film to the micromachine surface. This is most often accomplished through a process known as self-assembled monolayer (SAM) deposition.f Self-assembled monolayers are molecular films that spontaneously form on a (usually pretreated) surface upon exposure to a reactive precursor molecule. SAM precursors generally consist of three main parts a terminal group, a backbone, and a head group. Fig. 3 shows these parts on a model SAM... 

Choi and co-workers [579] studied the reactivity of vinyl-terminated self-assembled monolayers (SAMs) of undec-lO-ene-1-thiol on gold (see Fig. 6.32) toward olefin cross-metathesis (CM). Vinyl groups on SAMs were successfully converted into a, P-unsaturated carbonyl groups by CM with acrylic acid, methyl acrylate, and acrylamide. Result shows that various useful functional groups can be introduced to SAMs on gold (and other solid surfaces) by olefin CM and suggests an alternative to the S3mthesis of desired molecules in solution [579]. 

Hydrogels can absorb a significant amount of water and exhibit good flexibility, comparable to natural tissue, and these properties have led to many applications in the biotechnological and medical fields [59, 60]. To achieve the sustained release of hydrophobic compounds, Murakami and co-workers developed a tissue-adhesive hydrogel covalently bonded to a reactive polymeric micelle formed from aldehyde-terminated CHO-PEG-/>-PLA copolymers [61, 62]. Recently, several approaches have been proposed to fabricate materials in sheet form in order to maximise the surface area, e.g., a layer-by-layer sheet of polyelectrolytes [63-65], a self-assembled monolayer sheet [66] and an NP fused sheet [67-69]. 

Abstract In this contribution we review our recent progress in studies that aim at the understanding of the relationship between structure and surface reactivity of organic thin films on the one hand, and at the micro- and nanofabrication of bioreactive or biocompatible platforms on the other hand. Self-assembled monolayers (SAMs) of... 

---