Selecting disease-specific mimotopes

One of the first studies, Dybwad et al. [193] used panning of a type 8 nonapeptide library, alternating positive selection on pooled sera from a number of rheumatoid arthritis (RA) patients, and negative selection (see reservations about the effectivity of such an approach, above) on sera from a control group. The experiment was moderately successful, in that the enriched phage population contained five unrelated clones which could 

The isolation of disease-related antibodies specific for either melanoma [ 180], the autoimmune thyroid peroxidase antigen [174], or neutrophil antigen characteristic of ulcerative colitis [17] from nonnaive patient repertoire phage libraries has been reviewed above in the Panning on cells section. The isolation of anti-viral antibodies with diagnostic or therapeutic potential from antibody repertoire libraries has been described in earlier papers e.g. against HIV [197], hepatitis B virus [198] and human respiratory syncytial virus [199], 

The use of phage-display libraries to characterize a number of epitopes recognized by polyclonal autoimmune antibodies has also been reported for affinity purified anti-TNFa 

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