Mimotope selection

On the basis of these considerations, this technique has become a widely used selection method to identify new binding molecules [12-17], mimotopes [18-23], or to characterize protein ligand and/or protein protein interactions [24-37]. Moreover, attempts to optimize the selection process have resulted in the setting up of new biopanning procedures, which have been performed not only on purified targets [24-37], cells [38, 39], and tissues [40] (in vitro selection), but also on living animals [41] (in vivo selection). 

Tafi R, Bandi R, Prezzi C, Mordelli MU, Cortese R, Monaci P, Nicosia A, Identification of HCV core mimotopes Improved method for the selection and use of disease-related phage-displayed peptides, Biol. Chem., 378 495-502, 1997. 

On the basis of these considerations, this technique has become a widely used selection method to identify new binding molecules [12-17], mimotopes [18-23], or to characterize protein-ligand and/or protein-protein interactions [24-... 

Testing of each component of the library, one at a time, is the desired goal of any screening method but has constraints of time and resources. One way to increase the sample throughput is to perform several parallel analyses. An alternative approach to speed up the screening process is to reduce the number of samples for analysis by selecting only a random number of library members [87]. An improvement over this protocol is iterative deconvolution [88]. In one such approach, called the mimotope approach [89], a pool of compounds of soluble libraries is tested first based on this outcome, a smaller pool of compounds is synthesized and sublibraries are retested. The process is repeated until a compound with the highest activity is identified. 

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