Repertoire phage

The isolation of disease-related antibodies specific for either melanoma [ 180], the autoimmune thyroid peroxidase antigen [174], or neutrophil antigen characteristic of ulcerative colitis [17] from nonnaive patient repertoire phage libraries has been reviewed above in the Panning on cells section. The isolation of anti-viral antibodies with diagnostic or therapeutic potential from antibody repertoire libraries has been described in earlier papers e.g. against HIV [197], hepatitis B virus [198] and human respiratory syncytial virus [199],... 

Huse, W.D., Sastry, L., Iverson, S.A., Kang, A.S., Aldng-Mees, M., Burton, D.R., Benkovic, S.J., and Lerner, R.A. (1989) Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda. Science 246, 1275-1281. 

Krebber, A., S. Bornhauser, J. Burmester, A. Honegger, J. Willuda, H.R. Bosshard, and A. Pluckthun, Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system. J Immunol Methods, 1997. 201(1) 35-55. 

Waterhouse, P, Griffiths, A D., Johnson, K S., and Winter, G. (1993) Combinatorial infection and in vivo recombination, a strategy for making large phage antibody repertoires. Nucleic Acids Res 21,2265,2266. 

Geoffroy, F., Sodoyer, R., Aujame, L (1994) A new phage display system to construct multicombinatonal libraries of very large antibody repertoires Gene 151, 109-113... 

Immune libraries and evolutionary selection strategies intersect in the area of antibody humanization. Recently, we have extended the repertoire of methods available for the generation of therapeutic human antibodies by developing phage display strategies for the selection and humanization of antibodies from immune animals other than mice. Our aim here was to modify protein sequence, in some cases in a very radical way, and yet retain the function of the parental antibody. In the following discussion, we will focus on these novel approaches. 

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