Section adhesives

Irrrmunocytochemical techniques involve long incubation steps that can result in section detachment The use of HTAR techniques during which seaions are 

Several adhesives are available, including poly-L-lysine, 3-aminopropyltri-ethoxysilane (APES), Vectabond (Vector Laboratories Ltd.). Treated slides are also available commercially, as are electrostatically charged slides that are especially effective with fresh cell preparations. The most cost-effective method is to prepare adhesive coated slides in-house . APES (5) is the section adhesive of choice large numbers of slides can be prepared in batches, dried, and stored indefinitely without deterioration. The adhesive qualities are excellent and are vastly superior to those of poly-L-lysine, for example. 

Load glass microscope slides into metal slide carrier racks. 

Immerse in fresh acetone for 5 min, agitate the rack occasionally. 

Transfer to freshly made APES solution and allow to stand for 5 min, again with occasional agitation.  

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I. Bake the glass slide with tissue sections approximately 5°C above the melting point temperature of paraffin in the oven for 15 min for improving the tissue section adhesive. The melting point temperature of paraffin depends on the specific paraffin product used. 

Acrylic emulsions (or acrylic latices) are characterised as high-solids polymers having a film-forming temperature at or below room temperature (Lavelle, 1986). They have excellent hydrolysis resistance compared with other resins and are well suited as modifiers for portland cement mortars. Studies have shown that certain acrylic latices impart excellent workability at lower water demand, thin section adhesion and toughness, improved flexural strength and tensile strength and outstanding adhesion (Lavelle, 1983). 

Aramid cores are made from paper (typically 1.5 mil in thickness) comprising w-aramid floe and fibrids, similar to the papers used in electrical applications discussed in the previous section. Adhesive node lines are printed on paper sheets that are then stacked, pressed, and heated to cure the adhesive. The resulting block is expanded. The adhesive-free areas form the hexagonal cells of the honeycomb configuration. The core is dipped several times in an epoxy or phenolic resin solution until the desired density and mechanical property levels are reached. The core is then cut into slices of the desired thickness. Face sheets are glued to each side of the core. The most common face sheet today is a composite of carbon fiber and epoxy resin. 

Section adhesive, 1% aqueous gelatin or either Biobond (British BioCell International, Cardiff, Wales, UK) or Vectabond (Vector Labs Ltd., Bretton, Petaborough, UK). 

As already hinted in earlier sections, adhesion cannot simply be related to the surface wettability and the surface chemical stracture, for in most cases it involves the important effects of composition, morphology, and properties of the inteiphase region in an adhesive joint In the following, we discuss the role of plasma treaUnent for different material combinations. 

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