Secondary metabolite gene

Investigation of Cryptic Secondary Metabolite Gene Clusters Using Computational Analysis and Combinatorial Biosynthesis... 

The NRPS-based biosynthesis of the myxochelins - catecholate-type siderophores produced by a number of myxobacterial strains - has also been investigated. The biosynthetic pathway, the only known myxobac-terial secondary metabolite gene cluster involved in iron acquisition, has been identified in S. aurantiaca Sg al5, S. cellulosum So ce56, and M. xanthus DK1622 (unpublished data), and six genes are found to be involved... 

Pseudomonas fluorescens Pf5 is a plant commensal of the rhizosphere that suppresses the growth of various plant pathogens. The sequenced genome was found to harbor nine secondary metabolite gene clusters. Six of these could be attributed to known biosynthetic pathways for pyoluteorin (23) (Scheme 4),... 

Some of the functions of the S. coelicolor CYPs are in secondary metabolism operons, for example, CYP158A2 located downstream of a Type III polyketide synthase and CYP105N1 located downstream of a nonribosomal peptide synthase. These are currently subject to functional studies using the tools of gene disruption and metabolite/metabolome profiling, although not all the secondary metabolite genes identified... 

Figure 10.1 Architecture of Axpergillus secondary metabolite gene clusters. Shown arc the A. nidulans Sterigmatocystin (ST) (A) and Penicillin (PN) (B) gene dusters as well as the A. terreus Lov gene cluser (C). The rectangle indicates the portion of the Lov cluster that has been heterologously expressed in A. nidulans, as discussed in the text.

Figure lOJ Summary of secondary metabolite gene regulation via G protein-mediated signal transduction. The indicated positive and ne tive regulation (arrows and bars, respectively) has been found to be mediated transcriptionally, post-transcriptionally, or by both mechanisms, as described in the text. 

Baltz, R.H. (2010) Streptomyces and Sac-charopolyspora hosts for heterologous expression of secondary metabolite gene clusters. / Ind. Microbiol. BiotechnoL,... 

Gomez-Escribano, J.P. and Bibb, M.J. (2011) Engineering Streptomyces coelicolor for heterologous expression of secondary metabolite gene clusters. Microb. Biotechnol, 4 (2), 207 -215. 

Walton JD (2(X)0) Horizontal gene transfer and the evolution of secondary metabolite gene clusters in fungi an hypothesis. Fungal Genet Biol 30 167-171... 

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