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SDS-PAGE sodium dodecyl

SDS-PAGE sodium dodecyl suIfate-poIyacrylamide gel electrophoresis... [Pg.16]

Fig. 1.43 Strategies for protein identification. (A) 2D gel electrophoresis approach. (B) 2D liquid chromatography approach. lEF Isoelectric focusing, sex strong cation exchange column, RP reverse phase column, SDS-PAGE sodium dodecyl sulfate polyacrylamide gel electrophoresis. Fig. 1.43 Strategies for protein identification. (A) 2D gel electrophoresis approach. (B) 2D liquid chromatography approach. lEF Isoelectric focusing, sex strong cation exchange column, RP reverse phase column, SDS-PAGE sodium dodecyl sulfate polyacrylamide gel electrophoresis.
Subtilisin BPN was prepared through a series of protein purification steps applied to the fermentation broth. These steps included ultrafiltration ethanol precipitation DEAE (diethyl-aminoethyl) Tris Acryl batch anionic exchange SP (sulfopropyl) Tris Acryl column cationic exchange and, concentration with an Amicon stirred cell. The enzyme purity was determined to be -951 via spectroscopic assays that measure the ratio of active enzyme to total protein. In addition, purity was verified via HPLC and SDS-page (sodium dodecyl sulfate polyacrylamide gel electrophoresis). [Pg.227]

SDS-PAGE, sodium dodecyl sulphate-polyacrylamide gel electrophoresis HPLC, high performance liquid chromatography PCR, polymerase chain reaction IEF, isoelectric focusing HPSEC, high performance size-exclusion chromatography DNA, DNA-binding fluorochrome CPE, cytopathic effect Had, hemadsorption PMA, production of murine antibodies. [Pg.341]

SDS-PAGE sodium dodecyl sulphate polyacrylamide gel electrophoresis SERS surface enhanced Raman spectroscopy... [Pg.186]

Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE). Sodium dodecyl sulfate (SDS) gel electrophoresis systems are used to determine the number and size of protein chains or protein subunit chains in a protein preparation. Initially, the protein preparation is treated with an excess of soluble thiol (usually 2-mercaptoethanol) and SDS. Under these conditions, the thiol reduces all disulfide bonds (-S-S-) present within and/or between peptide units, while the SDS (an ionic or... [Pg.68]

PGC PPAR-gamma-coactivator SDS-PAGE sodium dodecyl sulphate - polyacrylamide... [Pg.949]

SDS/PAGE sodium dodecyl sulfate/polyacrylamide TOCSY total correlation spectroscopy... [Pg.244]

GRP 78 glucose-regulated protein of SDS-PAGE sodium dodecyl sulfate-poly-... [Pg.425]

SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) 725, 1563... [Pg.1879]

SDS-PAGE Sodium Dodecyl Sulfate Polyacryl Amide Gel Electrophoresis... [Pg.161]

The purpose below is not to describe methodologies for evaluating purity, but rather it is to use selected methodologies to demonstrate or to monitor the effectiveness of purification based on phase separation.The utilized methodologies are SDS PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and radiolabeling of contaminants. TTie lysed or ruptured cells of E. coli, transformed to produce the specific T,-type protein-based polymer, provide the impure reference state against which subsequent purification steps may be compared. [Pg.482]

Figure 7.3 K Ras4B C-terminal peptide was synthesized employing the trityl linker and after purification was reacted with a K-Ras thioester (K-Ras 1-174 MesNa) via EPL, yielding the semisynthetic K-Ras4B. Purity and identity of the purified protein were characterized by SDS-PAGE and MALDi-TOF. SDS-PAGE sodium dodecyl sulfate/polyacrylamide gel electrophoresis, MALDi-TOF matrix-assisted laser desorption ionization/time of flight. Figure 7.3 K Ras4B C-terminal peptide was synthesized employing the trityl linker and after purification was reacted with a K-Ras thioester (K-Ras 1-174 MesNa) via EPL, yielding the semisynthetic K-Ras4B. Purity and identity of the purified protein were characterized by SDS-PAGE and MALDi-TOF. SDS-PAGE sodium dodecyl sulfate/polyacrylamide gel electrophoresis, MALDi-TOF matrix-assisted laser desorption ionization/time of flight.
SDS-PAGE sodium dodecyl sulphate polyacrylamid gel electrophoresis PanijGroPBm I -dipahnitoylpnosphati lemanolamine... [Pg.1954]

SDS-PAGE Sodium dodecyl sulfate polyacrylamide gel electrophoresis SMP Submitochondrial particles... [Pg.530]

See other pages where SDS-PAGE sodium dodecyl is mentioned: [Pg.286]    [Pg.45]    [Pg.21]    [Pg.166]    [Pg.253]    [Pg.49]    [Pg.6279]    [Pg.252]    [Pg.19]    [Pg.49]    [Pg.745]    [Pg.290]    [Pg.73]    [Pg.780]    [Pg.1558]    [Pg.418]    [Pg.6278]    [Pg.292]    [Pg.120]    [Pg.236]    [Pg.276]    [Pg.1099]   


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SDS-PAGE

Sodium dodecyl sulfate SDS-PAGE)

Sodium dodecyl sulfate polyacrylamide gel electrophoresis, SDS-PAGE

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