Sodium dodecyl sulfate SDS-PAGE

Separation of proteins present in the IPG strip on a sodium dodecyl sulfate (SDS)-PAGE... 

Some argument remained until recently to cast doubt on the number of subunit types in the MoFe protein. Polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS-PAGE) on Cpl (Huang et al., 1973) and Kpl (Eady et ai, 1972) indicated the presence of two subunit types. However, Bulen (1976) cast doubt upon the interpretation of results obtained with SDS-PAGE when he showed that carboxymethylated Avl migrated as a single band whereas the untreated protein showed two types of subunits. Bulen attributed the appearance of two types of subunits to Fe associated... 

Most of the applications of HPLC for protein analysis deal with the storage proteins in cereals (wheat, corn, rice, oat, barley) and beans (pea, soybeans). HPLC has proved useful for cultivar identihcation, protein separation, and characterization to detect adulterations (illegal addition of common wheat flour to durum wheat flour) [107]. Recently Losso et al. [146] have reported a rapid method for rice prolamin separation by perfusion chromatography on a RP POROS RH/2 column (UV detection at 230nm), sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE), and molecular size determination by MALDl-MS. DuPont et al. [147] used a combination of RP-HPLC and SDS-PAGE to determine the composition of wheat flour proteins previously fractionated by sequential extraction. 

Sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) analysis and agarose gel electrophoresis equipment. 

Fig. 1. Sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) analysis of expression and purification of recombinant protein. Ten-microliter aliquots were withdrawn at each step of the purification and loaded on a 12% SDS-PAGE gel in a Mini Protean III cell gel electrophoresis unit (Bio-Rad). The detection was performed with Coomassie blue staining. MW, low range (14-98 kDa) molecular weight marker (Bio-Rad).

This protocol is for translating the proteins at preparative scale using 50-pL to 2-mL reaction volumes. The level of protein synthesis during a time course was analyzed by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) and is shown in Fig. 4. 

Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE). Sodium dodecyl sulfate (SDS) gel electrophoresis systems are used to determine the number and size of protein chains or protein subunit chains in a protein preparation. Initially, the protein preparation is treated with an excess of soluble thiol (usually 2-mercaptoethanol) and SDS. Under these conditions, the thiol reduces all disulfide bonds (-S-S-) present within and/or between peptide units, while the SDS (an ionic or... 

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