Sodium dodecyl sulphate polyacrylamide-PAGE

Molecular masses of the same enzymes of different species are different. Molecular mass of the laccase of Pleorotus ostreatus was found to be 66.8 kDa by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) [48]. Purified enzyme of T. versicolor having a single band with a molecular mass of 68 kDa was in the same range with the molecular weights of laccase isoforms isolated from 2,5-xylidine-induced cultures of T. versicolor [49]. 

Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE)... 

Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) represents the most commonly used analytical technique in the assessment of final product purity (Figure 3.31). This technique is well established and easy to perform. It provides high-resolution separation of polypeptides on the basis of their molecular mass. Bands containing as little as 100 ng protein can be visualized by staining the gel with dyes such as Coomassie blue. Subsequent gel analysis by scanning laser densitometry allows quantitative determination of the protein content of each band (thus allowing quantification of protein impurities in the product). 

Chemical purity. The purity of the protein product is analysed in comparison with a reference preparation by a suitable method, such as liquid chromatography, capillary electrophoresis or sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). 

SDS-PAGE, sodium dodecyl sulphate-polyacrylamide gel electrophoresis HPLC, high performance liquid chromatography PCR, polymerase chain reaction IEF, isoelectric focusing HPSEC, high performance size-exclusion chromatography DNA, DNA-binding fluorochrome CPE, cytopathic effect Had, hemadsorption PMA, production of murine antibodies. 

PL PVC Q RPC S SM SP SDS SDS-PAGE TEAE TAM Ve V0 vs Vt X y reversible complex ptotein-ligand poly vinyl chloride quaternary amine reverse phase chromatography Sulfonate Sulfomethyl Sulfopropyl sodium dodecyl sulphate sodium dodecyl sulphate - polyacrylamide gel electrophoresis Triethylaminoethyl Trimethylaminomethyl elution volume interstitial volume of the porous matrix total solvent volume within the pores total column volume solute concentration in the original solution solute concentration in the extract... 

SDS-PAGE sodium dodecyl sulphate polyacrylamide gel electrophoresis SERS surface enhanced Raman spectroscopy... 

PGC PPAR-gamma-coactivator SDS-PAGE sodium dodecyl sulphate - polyacrylamide... 

SDS-PAGE sodium dodecyl sulphate polyacrylamid gel electrophoresis PanijGroPBm I -dipahnitoylpnosphati lemanolamine... 

The bacteria were cultured at temperature 37°C and 42 C on Luria-Bertani medium supplemented with 50 pg/mL kanamycin. 0.5 mM IPTG was applied to induce synthesis of target enzymes in microbial cells. Process of PyrNPase and mutPyrNPase synthesis by E. coli was monitored by electrophoresis in polyacrylamide gel containing sodium dodecyl sulphate (SDS-PAGE). The percentage of enzymes of total cell proteins was calculated using TotalLab 120 software. 

Figure 10.6 shows sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) blots. On the left side the protein solution is shown after extraction, on the right side the fractionated proteins, pure cruciferin, and pure napin are shown. The cruciferin fraction has good emulsifying, film and gel formation properties, whereas the napin fraction is characterized by a high solnbility and foam stabilization properties. 

A, Absorption chi, chlorophyll car, carotenoid EET, excitonic energy transfer EF, exoplasmic fracture face EM, electron microscopy FWHM, full width at half maximum lEF, Isoelectric Focusing, LD, linear dichroism LHC, light harvesting complex PAGE, polyacrylamide gel electophoresis PF, protoplasmic fracture face PS, photosystem RC, reaction centre SDS, sodium dodecyl sulphate SSTT, single step transfer time. 

Abbreviations BSA, bovine serum albumin Chi, chlorophyll DCPIP, 2, 6-dichlorophenol indophenol DMBQ, 2, 6-dimethyl-l, 4-benzoquinone DTT, dithiothreitol Fd, ferredoxin Hepes, N-2-hydroxyethyl-piperazine N -2-ethane sulphonate LDAO, lauryldimethyl amine oxide Mes, 4-morpholine ethane sulphonic acid MV, methyl viologen PAGE, polyacrylamide gel electrophoresis PRuK, phosphoribulokinase PS, photosystem Ru5P, ribulose 5-phosphate SDS, sodium dodecyl sulphate TMPD, N, N, N, N -tetramethyl paraphenylene diamine TRX. thioredoxin... 

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