Competition-Based Screening

An alternative approach is competition-based screening. " A known medium-affinity binder (reporter ligand) is added to the solution of the target. If other ligands... 

Figure 7 Depiction of competition-based screening. Low- to medium-affinity binders are displaced from their binding site by higher affinity binders. In this experiment, only the signals of these low- to medium-affinity binders (reporter ligands) are monitored...

Colloidal carbon NPs have been used as labels in a rapid screening method based on an indirect competitive immunostrip test for detection of the pesticide thiabendazole in fruit juices after sample dilution (Blazcova et al., 2010). The strip-assay was based on... 

Fig. 1. Competition binding screens Bead/ligand compatibility test in early assay development. The beads PVT-WGA, Type A and Type B are based on polyvinyltoluene (PVT) not polyethyleneimine (PEI). PVT, polyvinyltoluene WGA, wheat germ agglutinin Ysi, yttrium silicate.

To test our new signal reagent based on GZ-11 the detection system was applied to two competitive-format immunoassays. These two assays (for atrazine and clenbuterol) normally use chromogenic detection systems. While these colorimetric assays may be adequate for laboratory use, chemiluminescent detection offers potential advantages in sensitivity and on site screening applications [33],... 

---