Screen-printed carbon electrodes SPCEs

R.M. Pemberton, J.P. Hart and T.T. Mottram, An assay for the enzyme lV-acetyl-/ -n-glucosaminidase (NAGase) based on electrochemical detection using screen-printed carbon electrodes (SPCEs), Analyst, 126 (2001) 1866-1871. 

This section outlines the development of genosensors on screen-printed carbon electrodes (SPCEs) for the identification of nucleic acid... 

The development and application of an electrochemical cell exclusively designed for disposable screen printed carbon electrodes (SPCE) suitable for simultaneous ECL and amperometric detection in sequential injection analysis (SIA) was described by Zen s group. The present flow system is user-friendly, requires less volume, and is easily operatable, since it has facility for PMT via a fiber optic facing the SPCE [89]. 

Moreover, due to some specific features of new forms of carbon, the spectrum of applications of traditional CPEs could be further extended. (This trend was proph-esized already in the mid-1990s [28, 29] in association with the rapid progress in the use of carbon inks for manufacturing of screen-printed carbon electrodes, SPCEs (see Chapter 12), that had for a certain time been considered as a derivative or successor of the CPEs themselves see also note in [5].)... 

Zhang et al. [18] developed nano-gold (ANP)-modified screen-printed carbon electrode (SPCE) for DNA detection. Based on the coulometric measurement of enzymatic silver (Ag) deposition, the target DNA was ultrasensitively detected in the 1990s, in the dynamic range of 3.0 X 10" to 1.0 X lO" " M (30 aM-lOOfM). The current-time curves during exhaustive electrolysis were undertaken before and after the target DNA hybridization (over 30 aM -lOOfM), as shown in Fig. 5 [18]. 

An electrochemical biosensor for the specific detection of short DNA sequences from the Escherichia coli E. coli) pathogen was suggested by Wang et al. The biosensor relied on the immobilization of the 25-mer oligonucleotide probe from the E. coli lacZ gene onto a screen-printed carbon electrode (SPCE). The hybridization event was monitored via chronopotentiometric detection of the Co(bpy)3 indicator. The biosensor detected 300 and 50 ng mL of E. coli DNA target with 20 and 30 min of hybridization time, respectively. Untreated environmental water samples were also used in order to present the applicability of the biosensor. 

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