Schematic internal reflectance fluorescence

Fig. 12. The figure at the left is the schematic illustration of laser induced total internal reflection fluorescence microscopy for the single molecule detection at the liquid-liquid interface. Abbreviations ND ND filter, 1/2 1/2 plate, M mirror, L lens, C microcell, O objective (60 x), F band path filter, P pinhole, APD avalanche photodiode detector. The figure at the right shows the composition of the microcell.

Fig. 15.1. Schematic representation of amyloid fibrils revealed by total internal reflection fluorescence microscopy, (a) The penetration depth of the evanescent field formed by the total internal reflection of laser light is 150nm for a laser light at 455 nm, so only amyloid fibrils lying parallel to the slide glass surface were observed. (b) Schematic diagram of a prism-type TIRFM system on an inverted microscope. ISIT image-intensifier-coupled silicone intensified target camera, CCD charge-coupled device camera...

FIGURE 1.5 Schematic diagram of flow injection analysis (FIA) system with immunoassay detection RIANA (abbreviation from River ANAlyser ) based on total internal reflection fluorescence with immobilized fluorescently labeled antibodies. The source of the excitation light is a He-Ne laser, and the collected fluorescent light is filtered and detected by photodiodes (PD). (Adapted from Rodriguez-Mozaz, S. et al. 2004. Biosens. Bioelectron. 19 633-640.)... 

Figure 1. Schematic of the optical fiber system. Excitation light is launched into the fiber. Due to the refractive index differences between the fiber core and cladding materials, the light is internally reflected and travels through the fiber with minimal loss (see inset). The emitted light is carried back from the fluorescent sensor located on the tip of the fiber to a CCD camera detector. Reprinted with permission from Science, 2000, 287, 451-452. Copyright 2000 AAAS.

FIGURE 10.5. The left figure shows the schematic illustration of laser-induced fluorescence microscopy under the total internal reflection for the detection of single Dil molecules at the dodecane-water interface. Abbreviations ND, ND filter XJ2, kl2 plate M, mirror L, lens C, microcell containing dodecane and aqueous phases O, objective (60 x ) F, bandpath filter P, pinhole APD, avalanche photodiode detector. The right portion of the figure shows the composition of the tnicrocell. 

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