Sanitation in Downstream Processes

Calculate the fractional residual capacity at the break point for the fixed-bed of Example 11.2. The fractional residual capacity of the adsorption zone can be approximated as 0.5. 

Estimate the residual capacity, when the interstitial velocity is doubled. It can be assumed that the averaged overall volumetric coefficient increases with the interstitial velocity to the power of 0.2. 

Since the fractional residual capacity of the adsorption zone is 0.5 and the bed height is 0.25 m, the residual capacity is given as (0.196x0.5) 

Two-thirds of the adsorption capacity is not utihzed at the break point in this case. 

1 A suspension of baker s yeast (p = 20kgm 3, p =0.0012 kg rn s ) is filtered with a dead-end filter (filter area = 1 m ) at a constant filtration pressure. Neglecting the resistance of the filtering medium, determine the filtration pressure difference AP to obtain 0.5 m of filtrate after 2.5 h. 

2 In the case where only the length of a gel chromatography column is doubled, how is the resolution of two solutes changed under the same operating conditions with the same packed beads  

5 When the height of the adsorbent bed is 50 cm under the same operating conditions given in Example 11.2, estimate the residual capacity. 

2 LeRoith, D., Shiloach, J., and Leahy, T.M. (eds) (1985) Purification of Fermentation Products - Application to Large-Scale Processes, ACS Symposium Series No. 

3 Scopes, R.K. (1994) Protein Purification — Principles and Practice, Springer-Verlag. 

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