Salmonella mutagenicity test water samples

Figure 4. Molecular weight determination of a drinking water concentrate with Sephadex LH20. Sampling, 10 -fold concentration of drinking water before and after chlorination (L5 mg/L of CI2, Meuse River source) on XAD-4/8, elution with DMSO (neutral fraction), and subsequent gel filtration were as described in Materials and Methods. After measuring the absorbance at 263 mm, the fractions were pooled as indicated. After dilution in water, the fractions were reconcentrated on XAD-4/8, eluted with DMSO, and assayed in the Salmonella mutagenicity test (strain TA98 S9).

$Figure 4. Molecular weight determination of a drinking water concentrate with Sephadex LH20. Sampling, 10 -fold concentration of drinking water before and after chlorination (L5 mg/L of CI2, Meuse River source) on XAD-4/8, elution with DMSO (neutral fraction), and subsequent gel filtration were as described in Materials and Methods. After measuring the absorbance at 263 mm, the fractions were pooled as indicated. After dilution in water, the fractions were reconcentrated on XAD-4/8, eluted with DMSO, and assayed in the Salmonella mutagenicity test (strain TA98 S9).$

Figure 10. Influence of a chlorine treatment on the mutagenic activity detectable with TA98NR and TA100NR strains. Sampling, 7000-fold concentration of water samples before and after a chlorine treatment (1.5 mg/L of Ch) on XAD-4/8, elution with DMSO (neutral fraction)> and subsequent testing of the DMSO concentrate in the Salmonella mutagenicity test were as described in Materials and Methods. Each value represents the average of three plates, and 0.2 mL of concentrate corresponds to 1.4 L of...

$Figure 10. Influence of a chlorine treatment on the mutagenic activity detectable with TA98NR and TA100NR strains. Sampling, 7000-fold concentration of water samples before and after a chlorine treatment (1.5 mg/L of Ch) on XAD-4/8, elution with DMSO (neutral fraction)> and subsequent testing of the DMSO concentrate in the Salmonella mutagenicity test were as described in Materials and Methods. Each value represents the average of three plates, and 0.2 mL of concentrate corresponds to 1.4 L of...$

Ames Test. Salmonella tryphimurium strains TA98 and TA100 were employed according to the Ames test (15, 16) to determine the mutagenic activity of the various water samples. Each DCM and MeOH extract was tested with and without S9 (microsomal fraction of activated rat liver with Arochlor 1254) activation. For each assay (16 assays per sampling point), the number of revertants per plate was plotted versus increasing volumes of water extracts injected. Slope values from linear regression of the dose-response curves were calculated and then used in the statistical analysis. [Pg.610]

Figure 4. Effects of variation of initial water content on mutagenicity of ground beef Samples are fried at 200 °C for 6 min per side and the basic fraction is tested for activity in the Salmonella mutagenesis assay. (The and O are data from two separate experiments.) The original fat-to-protein ratio of approximately 0.8 in the ground beef was maintained in these samples.

$Figure 4. Effects of variation of initial water content on mutagenicity of ground beef Samples are fried at 200 °C for 6 min per side and the basic fraction is tested for activity in the Salmonella mutagenesis assay. (The and O are data from two separate experiments.) The original fat-to-protein ratio of approximately 0.8 in the ground beef was maintained in these samples.$

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