Saccharomyces cerevisiae reproduction

To increase the activity and capability of reproduction of Saccharomyces cerevisiae (SC) Kabatov et al. [3.32] proposed the addition of 10 % skimmed milk, which has been saturated with Ar or N2. The freezing down to -25 °C was done under pressure and continued down to -55 °C. The freeze dried suspension did not change its quality during storage at +4 °C. 

Fungi are used extensively as model developmental systems in biochemistry and genetics. An early classical study of steroid-mediated morphogenesis involved the Oomycete Ac/i/ya ambisexualis (Raper 1939, 1940). The ascomycetous yeast Saccharomyces cerevisiae is the most extensively studied eukaryote in many areas of signal transduction (Kurjan 1993 Ferguson et al. 1994 Grishin et al. 1994 Schultz et al. 1995), especially as it relates to cell division and sexual reproduction. 

The overall life cycle of a particular yeast Saccharomyces (S.) cerevisiae, is summarised in Fig. S.6 which shows how it is possible for the cells to fuse to form various cell and spore types. The figure shows the possible types of reproduction in yeast. Generally, industrial strains of S. cerevisiae, brewers yeast, reproduce by budding/ fission processes and only sporulate under specialised conditions. However, many strains of yeast are capable of cell fusion to form spores or cells with increased genetic complements. Such strains have many sets of chromosomes and are termed polyploid. Active fermentation of industrial strains involves growth by mitotic division and nutrient depletion which results in stationary cells with little or no spore formation. 

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