Saccharomyces cerevisiae genome sequence

It should be possible to extend the DNA microarray-binding experiment to whole-genome analysis of transcription factor binding sites. The authors suggest that a microarray spotted with 12,000 one-kilobase sequences would span the entire Saccharomyces cerevisiae genome (Bulyk et al., 2001). Such an array could be used to characterize the sequence specificity of S. cerevisiae transcription factors. These experiments would be useful for predicting functions of previously uncharacterized transcription... 

Wolfsberg, T. Gabrielian, A. Campbell, M.J. Cho, R. Spouge, L Landsman, D. Candidate regulatory sequence elements for cell cycle-dependent transcription in saccharomyces cerevisiae. Genome Research 1999, 9, 775-792. 

The function of many noncoding RNAs (ncRNAs) depend on a defined secondary structure. RNAz detects evolutionarily conserved and thermodynamically stable RNA secondary structures in multiple sequence alignments and, thus, efficiently filters for candidate ncRNAs. In this chapter, we provide a step-by-step guide on how to use RNAz. Starting with basic concepts, we also cover advanced analysis techniques and, as an example for a large scale application, demonstrate a complete screen of the Saccharomyces cerevisiae genome. 

E2 are structurally and functionally diverse. Early biochemical work by using reticulocyte system revealed five distinct proteins with properties of E2. With the advent of genome sequencing, this observation has been corroborated. Even simple eukaryotes like yeast Saccharomyces cerevisiae) have 13 genes potentially encoding E2s. The number of E2s in mammals is estimated to be in the range of 25-30. 

Report of the first complete sequence of a eukaryotic genome, the yeast Saccharomyces cerevisiae. 

A collaboration of scientists reported sequencing of the complete genome of a complex organism. Saccharomyces cerevisiae, otherwise, known as baker s yeast. The achievement marked the complete sequencing of the largest genome to date more than 12 million base pairs of DNA. I... 

All fungal genomes archived encode at least one MCO of ca 700 residues that contains a carboxyl-terminal transmembrane domain this is in addition to the laccase(s) that many also produce. Most if not all of these membrane-associated MCOs are ferroxidases, and, hke Cp and Hp, play an essential role in the iron metabolism of these fungi. Three of these were represented in the sequences given in Figure 4 (FetSp, Fet5p and Fiol). Of these proteins, the most thoroughly characterized representative is the FetSp protein from baker s yeast, Saccharomyces cerevisiae. 

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