Saccharomyces cerevisiae cell ageing

Piton, M., Charpentier, C., and Troton, D. (1988). Cell wall and lipid changes in Saccharomyces cerevisiae during aging of champagne wine. Am. J. Enol. Vitic., 39, 221-226. 

Other vital stains take advantage of different cellular properties which can be correlated with cellular physiology Propidium Iodide, Ethidium Bromide, Ethidium Monoazide, Calcofluor White have been widely used to indicate the presence of dead eukaryotes or prokaryotes cells. 2-(p-iodophenyl-)3)(p-nitro-phenyl)-5-phenyl tetrazolium chloride (INT) belongs to a class of stains which can be used to determine if a cell or hyphal compartments [180] can maintain an internal reducing environment (Fig. 20a). There are, however, still a large debate about the reliability of those techniques, depending upon the cells under consideration [181]. Calcofluor (Aex = 380 nm, Aem 420 nm) is a specific cell wall stain which enables to counts buds scars on Saccharomyces cerevisiae [29] to estimate the age of a cell. 

As a whole, PolyPs in cells of eukaryotes are characterized by plural localization, depending on the cell age or environmental conditions. For example, cytochemical data on the localization of PolyPs in the cells of Saccharomyces cerevisiae are presented in Figure 5.2, according to Vofisek et al. (1982). Further studies of this problem may provide new data on the functions of these biopolymers. 

Fabrizio P, Longo VD (2003) The chronological life span of Saccharomyces cerevisiae. Aging Cell 2 73-81... 

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