Saccharid library

Another important discovery in the area of combinatorial synthesis with di- and tri-saccharides was made by Kahne and co-workers at Princeton [4c]. Kahne s approach utilized solid phase technology to synthesize a saccharide library (Scheme 3), which was especially challenging since, in order for a solid phase approach to be successful, bonds between monomers must be formed in high yields. This is not trivial since most high-yielding coupling reactions in carbohydrate chemistry are not general in nature [5]. 

Carbohydrate Microarrays as Essential Tools Saccharide libraries... 

Suitable testing methods in combination with synthetic nucleotide/ peptide/saccharide libraries enable investigations to be made on diverse biochemical issues in a more efficient manner. OSDB (http //www.orgsyn.org/) that compiles organic reactions and experimental procedures is a useful organic synthetic resource site. 

PoweU, A. K., Ahmed, Y. A., Yates, E. A., Turnbull, J. E. (2010). Generating heparan sulfate saccharide libraries for glycomics apphcations. Nature Protocols, 5(5), 821—833. 

Ishi-i T, Nakashima K, Shinkai S, Dceda A. Saccharide libraries as potential templates for regio- and chiroselective introduction of two fimctional groups into [60]fuUerene. J. Org. Chem. 1999 64 984-990. 

I c R, six glyc syl acceptors (monosaccharides) resin TentaGel (hydrophilic PS) Rj 12 glycosyl donors (mono- and disaccharides) library size 6x12x18=1296 Rj 18 acylating agents saccharides... 

Meyer et al. recently reported transfer NOE (nuclear overhauser effect) (trNOE) (88), which detects the strong negative trNOE effect of receptor-bound molecules compared to the weak, positive trNOE of unbound compounds without the need of receptor labeling the method was used to identify an E-selectin antagonist from an artificially assembled 10-member library of saccharides (89). 

A selection of other orthogonally protected saccharide intermediates with potential for library synthesis is shown in Fig. 11 [77]. 

Synsorb. Because several of these supports are also used in solid-phase synthesis, an advantage of using carbohydrate-displaying beads as model systems for polyvalent recognition events is that libraries of saccharides can be assembled. Therefore, structure-function relationships can be determined rapidly. 

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