Roxithromycin analysis

Konno, S., Asano, K., Kurokawa, M., Ikeda, K., Okamoto, K., and Adachi, K. (1994). Antiasthmatic activity of a macrolide antibiotic, roxithromycin Analysis of possible mechanisms in vitro and in vivo. Int. Arch. Allergy Immunol. 105, 308-316. 

Dubois et al. [59] determined the macrolides tylosin, tilmicoson, spiramycin, josamycin, and erythromycin in swine and bovine muscle, kidney and liver tissue, in bovine milk, and in hen eggs, using roxithromycin as IS. The method involves extraction in a Tris buffer, protein precipitation, SPE clean-up on a Oasis HLB cartridge, and LC-MS-MS analysis in SRM mode. All analytes were confirmed by four ions with an ion-ratio reproducibility ranging from 2.4 to 15%. The sample throughput is 50 samples per analyst per day. Draisci et al. [60] developed a confirmatory method for tylosin, tilmicosin, and erythromycin in bovine muscle, liver, and kidney. The quantification limits were 30, 20, and 50 pg/kg in mnscle, 40, 150, and 50 pg/kg in liver, and 40, 150, 80 pg/kg in kidney for tylosin, tilmicoson, and eiythromycin, respectively. Horie et al. [61] reported the multiresidne determination of erythromycin, oleandromycin, litasamycin, josamycin, mirosamycin, spiramycin, tilmicoson, and tylosin in meat and fish. The LOQ was 10 pg/kg in positive-ion LC-ESI-MS in SIM mode. 

Girault, J. P, Gharbi, J., Laoui, A., Artaud, 1., and Delaforge, M. (1989). Conformational analysis in solution of roxithromycin, a new macrolide antibiotic. Presented at 16th Int. Congr. Chemother. (June 11-16, Jerusalem). 

When combined with proton pump inhibitor (PPI) and other antimicrobials for the therapy of duodenal ulcer and chronic active gastritis, roxithromycin showed excellent activity in eradicating H. pylori. Eradication rates of 80-100% as determined by urease test, histologic analysis, and microbiologic cultures were achieved in various randomized open clinical trials [174-176]. However, lower rates of cure (50-67%) were found in larger randomized comparative studies [177,178]. 

Carbon, C., Hotton, J. M., Pepin, L. F., Wohlhuter, C., Souetre, E., Hardens, M., Lozet, H., and Riviera, M. (1996). Economic analysis of antibiotic regimens used in the treatment of pharyngitis A prospective comparison of azithromycin versus roxithromycin. J. Antimicrob. Chemother. 37, 151—161. 

Grgurinovich, N. Matthews, A, Analysis of erythromycin and roxithromycin in plasma or serum by high-performance liquid chromatography using electrochemical detection. J.Chromatogr., 1988,433, 298-304... 

Montenez JP, Van Bambeke F, Piret J, Brasseur R, Tulkens PM, Mingeot-Leclercq MP (1999) Interactions of macrolide antibiotics (erythromycin A, roxithromycin, erythromycylamine [Dmthromycin] and azithromycin) with phospholipids computer-aided conformation analysis and studies on acellular and cell culture models. Toxicol Appl Pharmacol 156 129-140 Morgan MY, Reshef R, Shah RR, Oates NS, Smith RL, Sherlock S (1984) Impaired oxidation of debrisoquine in patients with perhexiline liver injury. Gut 10 1057-1064 Morris AAM (1999) Mitochondrial respiratory chain disorders and the liver. Liver 19 357-368 Morris AAM, Taanman JW, Blake J, Cooper JM, Lake BD, Malone M, Love S, Clayton PT, Leonard JV, Schapira AHV (1998) Liver failure associated with mitochondrial DNA depletion. J Hepatol 28 556—563... 

Ding et al. described an automated on-line SPE-LC-MS/MS method for the determination of macrolide antibiotics, including erythromycin, roxithromycin, tylosin, and tilmicosin in environmental water samples. A Capcell Pak ME Ph-1 packed-column RAM was used as SPE column for the concentration of the analytes and clean-up of the sample. One millilitre of a water sample was injected into the conditioned SPE column, and the matrix was washed out with 3 ml high-purity water. By rotation of the switching valve (see Fig. 4.2), macrolides were eluted in the back-flush mode and transferred to the analytical column. The limits of detection and quantification obtained were 2-6 and 7-20 ng/1, respectively, which is suitable for trace analysis of macrolides. The intra- and inter-day precisions ranged within 2.9-12% and 3.3-8.9%, respectively. At the three fortification concentrations tested (20, 200, and 2000 ng/1), recoveries of macrolides ranged from 86.5% to 98.3%. 

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