Rosette labeling

Projection on (010) showing the two-dimensional network. Hydrogen bonds are represented by broken lines, and ring motifs A to F are shown in boldface type. The TPA2- ladders linking the rosette tapes are labeled (I) and (II). 

Scanning electron micrographs of mouse spleen lynqphocytes labeled for surface Ig molecules with latex spheres are illustrated in Figure 9 Mai r of the labeled cells (B-cells) were found to have microvilli-like structures latex markers were densely distributed over their cell surface and microvilli. Recent SEM studies indicate that human T-lymphocytes, which form rosettes with sheep blood cells, also exhibit numerous microvilli on their surface. ... 

Fig. 79. Photomicrograph of camera lucida drawing (inset) of anterogradely labelled axons and terminal boutons in the crus I ansiform lobule of rat cerebellum after cholera toxin (b fragment) injection into the contralateral ventral tegmental area. Arrows point to rosettes characteristic of mossy fiber endings in the granular layer (G). P, Purkinje cell layer WM, white matter. Scale bar = 40 ixm. Ikai et al. (1992).

Barmack et al. (1992a) noted a dense population of small ChAT-positive mossy fiber-like terminals in the tip of lobules IXa,b of rat cerebellum that are significantly smaller than the other ChAT positive rosettes (Fig. 83). This type of labelling was not described by Ojima et al. (1989) but could be observed in their Fig. 4. The source and characteristics of this peculiar population of terminals is still unknown. 

Fig. 199. Primary and secondary vestibulocerebellar mossy fiber projections in the rabbit, determined with antegrade axonal transport of [3H]leucine and WGA-HRP. Upper panels sagittal sections lower panels transverse sections through the caudal vermis. K196 ipsilateral distribution of fibers of the vestibular nerve. Gerrits et al., (1989) C2098 bilateral distribution of fibers from the medial vestibular nucleus (MV) K82 bilateral distributions of fibers from the superior vestibular nucleus (SV Thunnissen et al., 1989). Dense termination in the sagittal sections is indicated with heavy hatching, scattered labelled mossy fiber rosettes with light hatching and dots. Note similarity in the distribution of primary and secondary vestibulocerebellar projections.

Some CRF-positive mossy fibers can be labelled in monkeys Saimiri sciureus and Macaca fasciculara, Foote and Cha, 1988) but they seem to be less frequent than in other mammalian species. Some of these CRF-immunoreactive rosettes may originate as collaterals from the climbing fibers in these species. 

Figure 8.66 Projection along c showing the nearly planar guanidinium-carbonate (1 1) rosette network II at z = 1/2 in the crystal structure of 35. The atom types are differentiated by size and different grey scales, and N-H- O hydrogen bonds are represented by dotted lines. The layer is composed of independent carbonate ions [labeled by their central carbon atoms C(1) and C(3)[ andguanidinium ions [C(2) and C(4)[ in equal numbers. Note Mean atomic deviation from plane of one rosette = 0.36 A, and mean atomic deviation from plane of ribbon fragment [C(1), C(2), C(3), C(4)[ = 0.44 A]. Symmetry transformation a (1 — x, I + y, 1 - z)...

Figure 8,71 Projection diagram showing a portion of the guanidinium-boric acid-carbonate 1 2 1 rosette network viewed along the a axis at x = 1/2 in the crystal structure of 37. The atom types are differentiated by size and color, and N-H- O and O-H O hydrogen bonds are represented by broken lines. The layer is composed of independent guanidinium ion C(1) (labeled by the central carbon atom), boric acid B(1), and carbonate ion C(2). Symmetry transformations a (x, — y, z), b (1 — x, 1 — y, 1 — z), c (1 — x, j y, 2 — z) and d (1 X, —y, 2 + z). Mean atomic deviation from rosette plane [A], 0.12 A [B], 0.29 A (see colour plate section)...

Small parts like snaps/rivets, buttons, appliques, pompoms and fringes, bows and rosettes, dungaree clasps and sliders, zipper components, belt fasteners, toggles, decorative and functional loops, and decorative labels can cause choking hazards to a child. 

Kimura, S., Laosinchai, W., Itoh, T., Cui, X., Linder, C.R., Brown, R.M., 1999. Immunogold labeling of rosette terminal ceUulose-s3fnthesizing complexes in vascular plant. Plant Cell 11, 2075-2086. 

ICimura S., Laosinchai W., Itoh T, Cui X.J., Linder C.R., and Brown, Jr. R.M. 1999. Immunogold labeling of rosette terminal cellulose-synthesizing complexes in the vascular plant Vigna angularis. The Plant Cell 11 2075-2085. 

It is well known that the rosette and linear terminal complexes (TCs) can be observed by the freeze-fracture replication technique. The structures revealed by this technique are known as putative cellulose-synthesizing TCs. Kimura et al. (1999) demonstrated that TCs in vascular plants contain cellulose synthases using a novel technique of sodium dodecyl sulfate (SDS)-solubilized freeze fracture replica labeling (SDS-FRL). The localization of the cellulose synthase to the TC was accomplished almost 40 years after the hypothesis of Roelofsen (1958) in which he stated that enzyme complexes could be involved in cellulose biosynthesis. It has been more than 30 years since the discovery of the first TC by Brown, Jr. and Montezinos (1976) and in particular, 26 years after the discovery of rosette TCs in plants by Mueller and Brown, Jr. (1980). 

It was observed that 74% of gold particles were attached to rosettes or within 20 nm from the edges of rosette particles. Individual rosette was labeled with 1-4 gold particles, but usually with 1-2 gold particles (inset of Figure 14-3). 

---