Root exudates binding

As the separation characteristics of liquid chromatographic and electrophoretic techniques markedly differ from each other, combined methods using the advantages of both procedures have been successfully used for the analysis of flavonoids. Thus, the use of CZE-UV, HPTLC-UV and GC-MS for the measurement of flavonoids in seeds and root exudates of Lotus pedunculatus has been reported. The rooting solution and seed exudate were passed through cellulose acetate filters to bind the flavonoids. After extraction,... 

The Heavy Metal Binding Capacity (HMBC) test is a bioassay that helps to quickly determine metal bioavailability in aquatic environments. HMBC can also be applied to soils and to root exudates from aquatic and terrestrial plants. The HMBC test is based on MetPLATE, a bacterial toxicity test that selectively detects metal toxicity. 

Dousset, S., Morel, J.L., Jacobson, A. and Bitton, G. (2001) Copper binding capacity of root exudates of cultivated plants and associated weeds, Biology and Fertility of Soils 34, 230-234. 

Malinowski, D. P., Zuo, H., Beleski, D. P., and Alloush. G. A. (2004). Evidence for copper binding by extracellular root exudates of tall fescue but not perennial ryegrass infected with Neotyphodium spp. Endophytes. Plant Soil 267. 1-12. 

To understand the changes in metal fractionation within the rhizosphere, we need more insight into the key factors influencing metal fractionation. It has been assumed that the factors affecting metal fractionation and bioavailability in soil include root-induced pH changes, metal binding by root exudates, root-induced... 

Hatching of the parasitic nematode Globodera rostochiensis has been found to be restricted by low concentrations of LaClj (Atkinson and Ballantyne 1979). Fifty percent (50%) inhibition occurred at 83-100 (jiM La ", this appearing to be caused by a prevention of the binding and action of essential stimulators in the root exudate of host potato plants. Contradictory evidence (Clarke and Hennessy 1981) suggests that La " is, in fact, a moderately effective stimulator of hatching and than any apparent inhibition occurs only with high concentrations of LaClj. 

The translocation of BaP from roots to shoots should be low, due to BaP hydrophobicity (Figure 4), unless special molecules are produced by plants, able to increase the apparent aqueous solubility of hydrophobic pollutants, such as 2,3,7,8-TCDD-binding proteins, shown by Campanella and Paul (2000) for dioxin absorption by zucchini (Cucurbita pepo L.) may be B. curtipendula root exudates can enhance the acquisition of BaP, then accumulate it into their roots however, it could not translocate great amounts of BaP into their stems (Figure 4A). 

J. L. Morel, M. Mench, and A. Guckert, Measurement of Pb, Cu and Cd binding with mucilage exudates from maize (Zea mays L.) roots. Biol. Pertil. Soils 2 29 (1986). 

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