Ribonuclease RNase

LSZ lysozyme RNase ribonuclease aLA a -lactalbumin aLA(-Ca2+) Ca2+ depleted aLA BSA bovine serum albumin. 

List of Abbreviations PCR, polymerase chain reaction RT-PCR, reverse transcription polymerase chain reaction DNA, deoxyribonucleic acid RNA, ribonucleic acid RNase, ribonuclease mRNA, messenger RNA GABAa, y-aminobutyric acid type A cRNA, copy RNA dNTPs, deoxy nucleoside triphosphates MMLV, Mouse Moloney murine leukemia vims RT, reverse transcriptase bp, base pair Tm, melting temperature DEPC, diethylpyrocarbonate OD, optical density mL, milliliter SA-PMPs, streptavidin paramagnetic particles dT, deoxy thymidine DTT, dithiothreitol DNase, deoxyribonuclease RNasin, ribonuclease inhibitor UV, ultraviolet TBE, Tris-borate, 1 mM EDTA EDTA, ethylenediaminetetraacetic acid Buffer RET, guanidium thiocyanate lysis buffer PBS, phosphate buffered saline NT2, Ntera 2 neural progenitor cells... 

List of Abbreviations cDNA, complementary DNA ddH20, double-distilled H2O dNTP, deoxyribonu-cleotide triphosphate EDTA, ethylenediaminetetraacetic acid MgCl2, magnesium chloride mRNA, messenger ribonucleic acid NaOH, sodium hydroxide PCR, polymerase chain reaction qRT PCR, quantitative reverse transcriptase polymerase chain reaction RNase, ribonuclease RT PCR, reverse transcriptase polymerase chain reaction UTR, untranslated region... 

The effects of inhibitors and activators on RNases Ti, N1 Ui, U2, and T2 are summarized in Table IV (5, 7, 8), showing the characteristics of each RNase. Ribonuclease Ti is strongly inhibited by 10 3M Zn2+ RNases and T2 are inhibited about half but RNases N and U2 are not inhibited. Ribonuclease T, is also strongly inhibited by 10 3M Ag+, while all other RNases are inhibited about half. But 10 3 M Cu2+ strongly inhibits RNase T2 and only about half inhibits the other RNases. All five RNases are not inhibited by 10 2 M ethylenediaminetetraacetate (EDTA) and 10-3 M ICH2COOH. No metallic cofactor is required for their action. 

RNA ribonucleic acid mRNA messenger RNA rRNA ribosomal RNA scRNA small cytoplasmic RNA snRNA small nuclear RNA tRNA transfer RNA RNAse ribonuclease Ser serine T thymine Thr threonine... 

RNase (RNAse ribonuclease) An enzyme that cleaves RNA. routes of administration of drugs There are many different routes but common ones include intravascular injection or infusion (into the blood vessels, e.g. by drip, mainly intravenous (into veins) but sometimes intra-arterial (into arteries) intramuscular (injection into muscles) subcutaneous (injection beneath the dermis of the skin) intradermal (injection into the skin) transdermal (across the skin. e.g. from skin patches) topical (application to the skin or mucous membranes) per rectum (by an ointment or suppository into the rectum) intravaginally (by an ointment or pessary into the vagina) intrathecal (by injection into the subarachnoid space of the spinal cord) intranasally (often as a spray) orally (by mouth) inhalation. rRNA ribosomal RNA. 

RNase. Ribonuclease an enzyme which degrades RNA. It is ubiquitous in living organisms and is exceptionally stable. The prevention of RNase activity is the primary problem in handling RNA. 

Abbreviations CCK, cholecystokinin RNase. ribonuclease G-protein, guanine nucleotide binding protein GPCR, G-protein-coupled receptor. SDS sodium dodecylsulfate, CTAH. hexadecyltrimethyl ammonium hydroxide DMPC. di-myristoylphosphatidylcholine DPPC, di-palmitoylphosphatldylcholine CMC, critical micellar concentration SUV, small unilamellar vesicles CD, circular dichroism NMR, nuclear magnetic resonance hs-DC, high sensitivity differential scanning calorimetry IR-ATR, infrared attenuated total reflection spectroscopy NOE, nuclear Overhauser effect MD, molecular dynamics DMSO, dimethylsulfoxide TFE, trifluoroethanol for abbreviations of peptides see tables land 2, and fig. 11. 

PEP, phosphoenolpyruvate PFK, phosophofructokinase raR, DNA binding domain of retinoic acid recqrtor RNase, ribonuclease(s) ... 

Fig. 18.16 Separation of proteins on a mixed stationary phase. (Reproduced by permission of Elsevier Science Publishers BV from Z. el Rassi and C. Horvath, J. Chromatogr., 359, 255 (1986). Stationary phases (A) strong anion exchanger (Zorbax SAX-300) (B) strong cation exchanger (Zorbax SCX-300) (C) 1 1 mixture of A and B. Particle size, 7pm. Column, 8cm x 6.2 mm i.d. (A and B), 10cm X 4.6mm i.d. (C) mobile phase, 20mM tris-HCI (pH 7.0), gradient from 0 to 0.3 M sodium chloride in 40 min, 1.5 ml min (A and B), 1 ml min (C) UV detector, 280 nm. RNase = ribonuclease A CYT = cytochrome c CHY = a-chymotrypsinogen A LYS = lysozyme Hb = haemoglobin CON = conalbumin LAC A = j5-lactoglobulin A.

Pancreatic RNase, Ribonuclease I. Similar enzymes Venom RNase, Thiobacillus thioparus RNase,... 

Figure 5 Chromatograms of proteins on a silica-bound Cu(ll)-1DA column with different surface densities of chelated metal. Column, 5-pm IDA-Vydac in Cu(ll) form, 100x4.6 mm (a) light-metal load and (b) heavy-metal load 30 min linear gradient 0-0.5 M (NH4)2S04 and 1—20 mM glycine in 25 mM phosphate buffer, pH 6.0 flow rate, 1. OmL/min temperature, 25 C. Proteins TRI, trypsin inhibitor LAC, /Mactoglobulin A CHY, a-chymotrypsinogen A RNase, ribonuclease A LYS, lysozyme.

LTIC low temperature isotropic carbon MASIF surface forces adhesion method NMR nuclear magnetic resonance RNase ribonuclease... 

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