RNA binding domains

Nakagawa J, Nakagawa J, Moroni C. A 20-amino-acid autonomous RNA-binding domain contained in an enoyl-CoA hy-dratase. Eur J Biochem 1997 244 890-899. 

PUA Putative RNA-binding domain in PseudoUridine synthase and Archaeosine transglycosylase E(MFP)AB 5(5) 3(3) ... 

SI Ribosomal protein Sl-like RNA-binding domain E(MFP)AB 7(18) 6(14) 1AH9... 

Bycroft, M., Hubbard, T. J., Proctor, M., Freund, S. M., and Murzin, A. G. (1997). The solution structure of the SI RNA binding domain a member of an ancient nucleic acid-binding fold. Cell 88, 235-242. 

Zn-coordinating RNA-binding domain, in RAN-binding protein binds RAN-GDP. 

Nucleolin lacks a characteristic DNA binding domain (Ginisty et al, 1999). Its non-specific affinity for DNA is conferred by two different domains its four RNA binding domains, particularly the 3rd and the 4th ones, and its C-terminal GAR domain (Hanakahi et al, 1999 Sapp et al, 1989). Of importance, these properties were determined in vitro with the native protein purified from cell extracts or recombinant truncated proteins they are likely to be altered in vivo by interaction with other DNA binding factors (Dempsey et al, 1998) and/or by post-translational modifications. 

Allain FFI, Gilbert DE, Bouvet P, Feigon J (2000b) Solution structure of the two N-terminal RNA-binding domains of nucleolin and NMR study of the interaction with its RNA target [In Process Citation], J Mol Biol 303 227-241... 

Bourbon H, Bugler B, Caizergues-Ferrer M, Amalric F (1983) Role of phosphorylation on the maturation pathways of a 100 kDa nucleolar protein. FEBS Lett 155 218-222 Bourbon HM, Lapeyre B, Amalric F (1988) Structure of the mouse nucleolin gene. The complete sequence reveals that each RNA binding domain is encoded by two independent exons. J Mol Biol 200 627-638... 

Ginisty H, Amalric F, Bouvet P (2001) Two different combinations of RNA-binding domains determine the RNA binding specificity of nucleolin. J Biol Chem 276 14338-14343... 

R. H., Draper, D. E. The RNA-binding domain of ribosomal protein Lll recognizes an rRNA tertiary structure stabilized by both fhiostrepton and magnesium ion. Nucleic Adds Res 2000, 28, 1778—... 

Figure 10.4. Schematic representation of the Tat protein and its functional regions, highlighting the basic RNA binding domain. The secondary structure of its RNA target, TAR, is shown. Critical residues for Tat binding within the recognition domain (highlighted) are shown in bold.

In fragile X syndrome, triplet repeat expansions are responsible for the majority of cases. It should be noted, however, that any mutation that disrupts the functional activity of the FMRl protein could lead to a similar phenotype. This was in fact established when a patient with severe fragile X syndrome was found who did not have a triplet repeat expansion. Rather, this patient had a point mutation that changed a highly conserved amino acid within the RNA-binding domain of the FMRl protein (De Boulle et al., 1993 Siomi et al., 1994 Musco et... 

In addition to RNA-binding domains, the auxiliary domains are important components of the hnRNPs (Biamonti and Riva, 1994). Unlike RNA-binding domains, these domains are unstructured and have diverse sequences, making their classification difficult. The best characterized domain is the glycine-rich type found in hnRNPs Al and A2/B1. In hnRNP Al, the glycine-rich domain is localized near the C-terminus and is... 

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