Rhodospirillum rubrum, cell

Cross-sectional view of a cell of Rhodospirillum rubrum, a purple photosynthetic bacterium. A double-membrane system surrounds the cell. The inner membrane is extensively invaginated into tubules (arrows). These look circular when they are cut in cross section. (Courtesy of Dr. Gerald Peters.)... 

Non-Forster fluorescence quenching of trans-etiochlorin by magnesium oc-taethylporphine in phosphatidylcholine vesicles gives evidence for a statistical pair energy trap. Energy transfer also occurs in the excited singlet manifold of chlorophyll. " The photophysics of bis(chlorophyll)-cyclophanes, models of photosynthetic reaction centres, have been explored for use in artificial photosynthesis.Picosecond time-resolved energy transfer in phycobilosomes have also been studied with a tunable laser. The effect of pH on photoreaction cycles of bacteriorhodopsin, " the fluorescence polarization spectra of cells, chromatophores, and chromatophore fractions of Rhodospirillum rubrum, and a brief review of the mechanism and application of artifical photosynthesis are all relevant to the subject of this Chapter. 

Schwerzmann RU and Bachofen R (1989) Carotenoid profiles in pigment-protein complexes of Rhodospirillum rubrum. Plant Cell Physiol 30 497-504... 

Barber, J. (1982). Influence of surface charges on thylakoid structure and function. Ann. Rev. Plant Physiol. 33 261-295 Loach, P.A., Parkes, P.S., Bustamante, P. (1984). Regulation of photosynthetic unit structure in Rhodospirillum rubrum whole cells. In Sybesma C. (ed) Adv. Photosynth. Res. vol II. 189-197 Holmes, N.G. and Allen, J.F. (1986). Protein phosphorylation as a control for excitation energy transfer in Rhodospirilium rubrum. FEBS Lett. 200 144-148... 

Rhodospirillum rubrum G9, a carotenoidless mutant, was grown photosynthetically at 32 Cells were harvested in... 

Culture of Rhodospirillum rubrum R. rubrum was cultured in Ortnerod medium(4) with na-late as C-source at pH 6.2-6.6, 28-30 C and under 27 8 uraol/S/M incandescent light and the cells were collected log phase The condition with COp was the same as without except 25tC02+98ji H2 was bubbled into the medium and malate omitted. 

Boatman, E.S. (1964) Observations on the fine structure of spheroplasts of Rhodospirillum rubrum. J. Cell Biol., 20, 297-311. 

Solvent extraction method is one of the oldest techniques adopted for extraction of PHB. The mechanism of action of solvent is that it modifies the cell membrane permeability and then solubilize PHB. This process was first adopted by Lemoigne and Baptist for the extraction of PHB from B. megaterium and Rhodospirillum rubrum Several solvent mixtures were tried for the separation of PHB from the solvent by solvent evaporation or by precipitation in a nonsolvent. 

The role, synthesis and depolymerization of PHB in the purple bacterium Rhodospirillum rubrum have been extensively investigated in the past [7, 22, 40]. Now, R. rubrum has recently been used by Ulmer et al. [112] to synthesize unusual polymers containing 3HB, 3HV, and 3-hydroxy-4-pentenoate (3H4PE) repeating units from both 4-pentenoic acid and pentanoic acid. Long growth times and low cell and polymer yields were observed. 

We have isolated reaction centers from Rhodospirillum rubrum G- 9 cells labeled with the isotope 39-Fe. On LDS-polyacrylamide electrophoresis the preparation shows the typical three bands H, M and L and some minor contaminations (Figure 1). The specific staining technique for c-type cytochromes gave no positive reaction, indicating that there is no such cytochrome among the contaminations (datanot shown). 

REGULATION OF PHOTOSYNTHETIC UNIT STRUCTURE IN RHODOSPIRILLUM RUBRUM WHOLE CELLS... 

---