Polyacrylamide gels for electrophoresis

Jackson, P., The use of polyacrylamide-gel electrophoresis for the high-reso-lution separation of reducing saccharides labeled with the fluorophore 8-ami-nonaphthalene-l,3,6-trisulfonic acid. Detection of picomolar quantities by an imaging system based on a cooled charge-coupled device, Biochem. ]., 270, 705, 1990. 

Schagger, H. and G. von Jagow (1987) Tricine-sodium dodecyl sulfate polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem., 166 368-379... 

TRICINE-SDS-Polyacrylamide Gel Electrophoresis for Proteins and Oligopeptides in the Range of 1000-50 000 Daltons... 

Proteomics Chromatographic Fractionation Prior to Two-Dimensional Polyacrylamide Gel Electrophoresis for Enrichment of Low-Abundance Proteins to Facilitate Identification by Mass Spectrometric Methods... 

Smith, B. J., SDS-polyacrylamide gel electrophoresis for N-terminal protein sequencing, in Protein Sequencing Protocols, Smith, B. J., Ed., Humana Press, Totowa, NJ, chap. 2, 1997, 17-24. 

Using terminal deoxynucleotidyltransferase -1- [a- P]ddATP, a 36-nucleotide oli-godeoxyribonucleotide containing uridine at position 21 is labeled at its 3 -end and the product is subjected to 20% denaturing polyacrylamide gel electrophoresis for... 

Dunn, M. J. and A. Gorg. 2001, Two-dimensional Polyacrylamide Gel Electrophoresis for Proteome Analysis. In Proteomics, From Protein Sequence to Function. S. R. Pennington and M. J. Dunn (eds.),), BIOS Scientific Pubhshers Ltd, pp. 43-63. 

Schagger, H., and Jagow, G. (1987). Tricine-sodium-dodecylsulfate Polyacrylamide Gel Electrophoresis for the Separation of Proteins in the Range from 1-100 kDa, Anal. Biochem. 166 368-379. 

Thylakoids were isolated as described in 5. Quantification of the apopeptides of LHCII was done by two-dimensional gel electrophoresis. For the first dimension chlorophyll-protein complexes were resolved by mild SDS-polyacrylamide gel electrophoresis. For the second dimension the green band corresponding to LHCII was excised and reelectrophoresed under denaturing conditions (5). 

The analysis of proteins falls under the umbrella of proteomics. For years, the field of proteomics rehed on two-dimensional polyacrylamide gel electrophoresis for the separation of complex protein mixtures. With the drive to produce methods with more selective and structural methods, HPLC with new column technology combined with the mass spectrometer has revolutionized the analysis of proteins. The quality and quantity of information generated by this approach has given rise to a... 

However, while the ultracentrifuge is no longer heavily used in biochemistry (because of the development of sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis for the estimation of molecular weight, see Chapter 13), it has become an important tool for the study of dimensions of synthetic polymers in solution. 

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