Rhodobacter sphaeroides growth

OH 0 j—y N-(3 -hydroxy-7-c w-tetradecenoyl)-L-homoserine lactone, 7A HtDHL or 7A, 3-OH, C4-HSL Rhizobium leguminosarum Rhodobacter sphaeroides Nodulation, growth inhibition, expression of rhizosphere genes, plasmid transfer Community escape... 

Growth and Utilization of Acetic acid by Rhodobacter sphaeroides IL106... 

Growth and utilization of acetic acid by Rhodobacter sphaeroides IL106 was investigated when ammonium was added as a nitrogen source. As shown in Fig. 3, growth (OD and dry cell) was attained up to about 3 g/L after 2 days culture. And acetic acid was consumed by these cells together with the drastic consumption of phosphate. Ammonium and nitrate consumed. It was confirmed that this bacteria consume acetic acid in anaerobic digestion liquor sediment mud and produced cells under 3 % NaCl concentration condition and with some other substances such as trace amount of heavy metals. 

Yakunin, A.F., Tsygankov, A.A., Troshina, O.Yu., Gogotov, I.N. (1991). Growth and nitrogenase activity of continuous cultures of the purple bacteria Rhodobacter capsulatus and Rhodobacter sphaeroides depending on the presence of Mo, V, and W in the medium. Mikrobiologiya (Russ.) 60, 41-46. 

Bebien, M., Chauvin, J. P., Adriano, J. M., Grosse. S.. and VeiTn glio. A. (2001). Effect of selenite on growth and protein synthesis in the phototrophic bacterium Rhodobacter sphaeroides. Appl. Envir. Microbiol. 61, 4440—4447. 

Eichenberger W, Boschetti A and Michel HP (1986) Lipid and pigment composition of a chlorophyll h-deficient mutant of Chlamydomonas reinhardttii. Physiol Plant 66 589-594 Ermler U, Fritzsch G, Buchanan SK and Michel H (1994) Structure of the photosynthetic reaction centre from Rhodobacter sphaeroides at 2,65 A resolution Cofactors and protein-cofactor interactions. Structure 2 925-936 Foidl M, Golecki JR and Oelze J (1997) Phototrophic growth and chlorosome formation in Chloroflexus aurantiacus under conditions of carotenoid deficiency. Photosynth Res 54 219-226... 

The ubiquinol-cytochrome c oxidoreductase (cytochrome bc complex) of Rhodobacter sphaeroides is an integral component of the intracytoplasmic membrane (ICM) and functions in light-driven cyclic electron flow and the conservation of radiant energy as an electrochemical proton gradient. Previous studies on the assembly of electron transfer constituents in/ , sphaeroides have demonstrated that complete cycles of electron flow do not occur merely upon insertion of newly synthesized reaction centers at sites of initiation of ICM growth, but instead, subsequent synthesis and assembly of redox centers of the be complex are required [1]. To further characterize the assembly process and for detailed structural investigations, the complex was purified and antibodies were raised against the isolated polypeptide constituents. In this report, results on the localization and levels of the feCj complex in various membrane fractions are presented a detailed description of the structurd work will appear elsewhere [2]. 

Rhodobacter sphaeroides wild type and mutant strain GA and Rhodo-spirillum rubrum wild type and mutant strain G9 were grown in Sistroms medium in 250ml flasks at 50 W.m" and 30 C or in a fermentor at 200 W.m and 30 C. For the measurements cells from the exponential growth phase were centrifuged and resuspended in buffer (MOPS, pH 7.0) or fresh Sistrom medium (pH 6.8). 

Barz WP, Vermeglio A, Francia F, Venturoli G, Melandri BA and Oesterhelt D. Role of the PufX protein in photosynthetic growth of Rhodobacter sphaeroides. 2. PufX is required for the efficient ubiquinone/ubiquinol exchange between the reaction centre Qb site and the cytochrome bci complex. Biochemistry 1995a 34 15248-15258. 

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