RFLPs linkage

Lander, E.S. and Botstein, D. (1989) Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 121, 185-199. 

Linkage analysis by studying RFLP patterns in two or more generations of family members afflicted with a disease is useful for genetic counseling for the prediction of inherited disease, in spite of its limitations, such as the possibility of genetic recombination or the presence of more than one gene locus in different family members. 

Indirect testing approaches are usually applied in those genetic disorders in which the defective gene site is unknown. These tests rely on RFLP probability linkages from family studies to evaluate disease and carrier status. 

Fig. 3. Linkage analysis of selected RFLP markers associated with neurofibromatosis-1 (NFl) on chromosome 17.

Restriction fragment length polymorphisms (RFLPs) were the first type of molecular markers used in linkage studies. RFLPs arise because mutations can create or destroy the... 

There has been few studies of MHC in other avian species, but there is evidence for linkage between MHC and blood groups in turkeys and ring-necked pheasants (Lamont Dietert, 1990). Four turkey class II genotypes have been identified, using RFLP and a domestic fowl DNA probe. Turkey erythrocytes appear either not to express class I proteins, or to express only very low levels (Emara et al, 1992). At least three class II B haplotypes and, therefore, six genotypes exist in ring-necked pheasant (Wittzell et al, 1994). 

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